| Title : A stereochemical test of a proposed structural feature of the nicotinic acetylcholine receptor - Hanek_2008_J.Am.Chem.Soc_130_13216 |
| Author(s) : Hanek AP , Lester HA , Dougherty DA |
| Ref : J Am Chem Soc , 130 :13216 , 2008 |
| Abstract : Understanding the gating mechanism of the nicotinic acetylcholine receptor (nAChR) and similar channels constitutes a significant challenge in chemical neurobiology. In the present work, we use a stereochemical probe to evaluate a proposed pin-into-hydrophobic socket mechanism for the alphaVal46 side chain of the nAChR. Utilizing nonsense suppression methodology we incorporated isoleucine (Ile), O-methyl threonine (Omt) and threonine (Thr) as well as their side chain epimers (the allo counterparts). Surprisingly, our results indicate that only the pro-S methyl group of the alphaVal46 side chain is sensitive to changes in hydrophobicity, consistent with the precise geometrical requirements of the pin-into-socket mechanism. |
| ESTHER : Hanek_2008_J.Am.Chem.Soc_130_13216 |
| PubMedSearch : Hanek_2008_J.Am.Chem.Soc_130_13216 |
| PubMedID: 18781739 |
Hanek AP, Lester HA, Dougherty DA (2008)
A stereochemical test of a proposed structural feature of the nicotinic acetylcholine receptor
J Am Chem Soc
130 :13216
Hanek AP, Lester HA, Dougherty DA (2008)
J Am Chem Soc
130 :13216