| Title : Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58 - Hasan_2011_Appl.Environ.Microbiol_77_1881 |
| Author(s) : Hasan K , Fortova A , Koudelakova T , Chaloupkova R , Ishitsuka M , Nagata Y , Damborsky J , Prokop Z |
| Ref : Applied Environmental Microbiology , 77 :1881 , 2011 |
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Abstract :
We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters. |
| PubMedSearch : Hasan_2011_Appl.Environ.Microbiol_77_1881 |
| PubMedID: 21193677 |
Hasan K, Fortova A, Koudelakova T, Chaloupkova R, Ishitsuka M, Nagata Y, Damborsky J, Prokop Z (2011)
Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58
Applied Environmental Microbiology
77 :1881
Hasan K, Fortova A, Koudelakova T, Chaloupkova R, Ishitsuka M, Nagata Y, Damborsky J, Prokop Z (2011)
Applied Environmental Microbiology
77 :1881