| Title : Genome structure of pTi-SAKURA (I): strategy for DNA sequencing of a Japanese cherry-Ti plasmid - Hattori_1997_Nucleic.Acids.Symp.Ser_37_159 |
| Author(s) : Hattori Y , Suzuki K , Uraji M , Ohta N , Katoh A , Yoshida K |
| Ref : Nucleic Acids Symp Ser , 37 :159 , 1997 |
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Abstract :
We isolated a plasmid from a bacterium Agrobacterium tumefaciens, which had been found in a crown gall tumor on a Japanese cherry tree SAKURA and designated it pTi-SAKURA. For complete DNA sequencing, we constructed a DNA library in lambda phage vector and developed a sequencing method by primer walking with long PCR and a PCR subcloning technique for long insert DNA. |
| PubMedSearch : Hattori_1997_Nucleic.Acids.Symp.Ser_37_159 |
| PubMedID: 9586048 |
| Gene_locus related to this paper: agrra-TIORF87 |
| Gene_locus | agrra-TIORF87 |
Hattori Y, Suzuki K, Uraji M, Ohta N, Katoh A, Yoshida K (1997)
Genome structure of pTi-SAKURA (I): strategy for DNA sequencing of a Japanese cherry-Ti plasmid
Nucleic Acids Symp Ser
37 :159
Hattori Y, Suzuki K, Uraji M, Ohta N, Katoh A, Yoshida K (1997)
Nucleic Acids Symp Ser
37 :159