| Title : Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis - Ho_2023_Chem.Commun.(Camb)_59_8234 |
| Author(s) : Ho YTC , Kaczmarski JA , Tailhades J , Izore T , Steer DL , Schittenhelm RB , Tosin M , Jackson CJ , Cryle MJ |
| Ref : Chem Commun (Camb) , 59 :8234 , 2023 |
|
Abstract :
Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes. |
| PubMedSearch : Ho_2023_Chem.Commun.(Camb)_59_8234 |
| PubMedID: 37310188 |
| Inhibitor | Phosphopantetheine |
Ho YTC, Kaczmarski JA, Tailhades J, Izore T, Steer DL, Schittenhelm RB, Tosin M, Jackson CJ, Cryle MJ (2023)
Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis
Chem Commun (Camb)
59 :8234
Ho YTC, Kaczmarski JA, Tailhades J, Izore T, Steer DL, Schittenhelm RB, Tosin M, Jackson CJ, Cryle MJ (2023)
Chem Commun (Camb)
59 :8234