Hui_2011_Insect.Mol.Biol_20_507

Acetylcholinesterase (AChE, EC 3.1.1.7) is a key enzyme in terminating synaptic transmission. We knocked down the expression of Csace1 or Csace2 using chemically synthesized small interfering RNAs (siRNAs) designed from divergent regions. The mRNA abundance of the two ace genes was reduced to 50-70% of control levels. The enzyme activities were decreased to 40-70%. Silencing of Csace1 or Csace2 resulted in a ~25% mortality rate. Knockdown of Csace1 had major effects on larval growth inhibition and resulted in reduced larval weight and length, malformation and motor disability, whereas silencing of Csace2 had only minor effects. These results suggested that both AChE-1 and AChE-2 have important roles in maintaining life in this insect and indicated that AChE-1 might have nontypical functions in regulating larval growth and motor ability.