Hwang_2004_Biotechnol.Lett_26_603

Reference

Title : Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization - Hwang_2004_Biotechnol.Lett_26_603
Author(s) : Hwang S , Ahn J , Lee S , Lee TG , Haam S , Lee K , Ahn IS , Jung JK
Ref : Biotechnol Lett , 26 :603 , 2004
Abstract :

A cellulose-binding domain (CBD) fragment of a cellulase gene of Trichoderma hazianum was fused to a lipase gene of Bacillus stearothermophilus L1 to make a gene cluster for CBD-BSL lipase. The specific activity of CBD-BSL lipase for oil hydrolysis increased by 33% after being immobilized on Avicel (microcrystalline cellulose), whereas those of CBD-BSL lipase and BSL lipase decreased by 16% and 54%, respectively, after being immobilized on silica gel. Although the loss of activity of an enzyme immobilized by adsorption has been reported previously, the loss of activity of the CBD-BSL lipase immobilized on Avicel was less than 3% after 12 h due to the irreversible binding of CBD to Avicel.

PubMedSearch : Hwang_2004_Biotechnol.Lett_26_603
PubMedID: 15168862

Related information

Citations formats

Hwang S, Ahn J, Lee S, Lee TG, Haam S, Lee K, Ahn IS, Jung JK (2004)
Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization
Biotechnol Lett 26 :603

Hwang S, Ahn J, Lee S, Lee TG, Haam S, Lee K, Ahn IS, Jung JK (2004)
Biotechnol Lett 26 :603