Hwang_2023_Int.J.Mol.Sci_24_12022

Reference

Title : Structural and Biochemical Insights into Bis(2-hydroxyethyl) Terephthalate Degrading Carboxylesterase Isolated from Psychrotrophic Bacterium Exiguobacterium antarcticum - Hwang_2023_Int.J.Mol.Sci_24_12022
Author(s) : Hwang J , Yoo W , Shin SC , Kim KK , Kim HW , Do H , Lee JH
Ref : Int J Mol Sci , 24 :12022 , 2023
Abstract :

This study aimed to elucidate the crystal structure and biochemically characterize the carboxylesterase EaEst2, a thermotolerant biocatalyst derived from Exiguobacterium antarcticum, a psychrotrophic bacterium. Sequence and phylogenetic analyses showed that EaEst2 belongs to the Family XIII group of carboxylesterases. EaEst2 has a broad range of substrate specificities for short-chain p-nitrophenyl (pNP) esters, 1-naphthyl acetate (1-NA), and 1-naphthyl butyrate (1-NB). Its optimal pH is 7.0, losing its enzymatic activity at temperatures above 50 degreesC. EaEst2 showed degradation activity toward bis(2-hydroxyethyl) terephthalate (BHET), a polyethylene terephthalate degradation intermediate. We determined the crystal structure of EaEst2 at a 1.74 A resolution in the ligand-free form to investigate BHET degradation at a molecular level. Finally, the biochemical stability and immobilization of a crosslinked enzyme aggregate (CLEA) were assessed to examine its potential for industrial application. Overall, the structural and biochemical characterization of EaEst2 demonstrates its industrial potency as a biocatalyst.

PubMedSearch : Hwang_2023_Int.J.Mol.Sci_24_12022
PubMedID: 37569396
Gene_locus related to this paper: human-DPP9 , exiab-k0afs0

Related information

Substrate BHET
Gene_locus BHET    human-DPP9    exiab-k0afs0
Structure BHET    human-DPP9    exiab-k0afs0    8HEA

Citations formats

Hwang J, Yoo W, Shin SC, Kim KK, Kim HW, Do H, Lee JH (2023)
Structural and Biochemical Insights into Bis(2-hydroxyethyl) Terephthalate Degrading Carboxylesterase Isolated from Psychrotrophic Bacterium Exiguobacterium antarcticum
Int J Mol Sci 24 :12022

Hwang J, Yoo W, Shin SC, Kim KK, Kim HW, Do H, Lee JH (2023)
Int J Mol Sci 24 :12022