Iermak_2021_Catalysts_11_5

Reference

Title : Description of Transport Tunnel in Haloalkane Dehalogenase Variant LinB D147C+L177C from Sphingobium japonicum - Iermak_2021_Catalysts_11_5
Author(s) : Iermak I , Degtjarik O , Havlickova P , Kuty M , Chaloupkova R , Damborsky J , Prudnikova T , Smatanova IK
Ref : Catalysts , 11 :5 , 2021
Abstract :

The activity of enzymes with active sites buried inside their protein core highly depends on the efficient transport of substrates and products between the active site and the bulk solvent. The engineering of access tunnels in order to increase or decrease catalytic activity and specificity in a rational way is a challenging task. Here, we describe a combined experimental and computational approach to characterize the structural basis of altered activity in the haloalkane dehalogenase LinB D147C+L177C variant. While the overall protein fold is similar to the wild type enzyme and the other LinB variants, the access tunnels have been altered by introduced cysteines that were expected to form a disulfide bond. Surprisingly, the mutations have allowed several conformations of the amino acid chain in their vicinity, interfering with the structural analysis of the mutant by X-ray crystallography. The duration required for the growing of protein crystals changed from days to 1.5 years by introducing the substitutions. The haloalkane dehalogenase LinB D147C+L177C variant crystal structure was solved to 1.15 A resolution, characterized and deposited to Protein Data Bank under PDB ID 6s06

PubMedSearch : Iermak_2021_Catalysts_11_5
PubMedID:
Gene_locus related to this paper: sphpi-linb

Related information

Gene_locus sphpi-linb
Structure sphpi-linb    6S06

Citations formats

Iermak I, Degtjarik O, Havlickova P, Kuty M, Chaloupkova R, Damborsky J, Prudnikova T, Smatanova IK (2021)
Description of Transport Tunnel in Haloalkane Dehalogenase Variant LinB D147C+L177C from Sphingobium japonicum
Catalysts 11 :5

Iermak I, Degtjarik O, Havlickova P, Kuty M, Chaloupkova R, Damborsky J, Prudnikova T, Smatanova IK (2021)
Catalysts 11 :5