Ishiguro_2001_Plant.Cell_13_2191

Reference

Title : The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis - Ishiguro_2001_Plant.Cell_13_2191
Author(s) : Ishiguro S , Kawai-Oda A , Ueda J , Nishida I , Okada K
Ref : Plant Cell , 13 :2191 , 2001
Abstract :

The Arabidopsis mutant defective in anther dehiscence1 (dad1) shows defects in anther dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1-specific manner, and DAD1-green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::beta-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.

PubMedSearch : Ishiguro_2001_Plant.Cell_13_2191
PubMedID: 11595796
Gene_locus related to this paper: arath-PLA11

Related information

Gene_locus arath-PLA11
Family arath-PLA11    Plant_phospholipase

Citations formats

Ishiguro S, Kawai-Oda A, Ueda J, Nishida I, Okada K (2001)
The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis
Plant Cell 13 :2191

Ishiguro S, Kawai-Oda A, Ueda J, Nishida I, Okada K (2001)
Plant Cell 13 :2191