Title : Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity - Janockova_2016_Mol.Biosyst_12_2910 |
Author(s) : Janockova J , Zilecka E , Kasparkova J , Brabec V , Soukup O , Kuca K , Kozurkova M |
Ref : Mol Biosyst , 12 :2910 , 2016 |
Abstract :
In this paper, we describe the biochemical properties and biological activity of a series of cholinesterase reactivators (symmetrical bisquaternary xylene-linked compounds, K106-K114) with ctDNA. The interaction of the studied derivatives with ctDNA was investigated using UV-Vis, fluorescence, CD and LD spectrometry, and electrophoretic and viscometric methods. The binding constants K were estimated to be in the range 1.05 x 10(5)-5.14 x 10(6) M(-1) and the percentage of hypochromism was found to be 10.64-19.28% (from UV-Vis titration). The used methods indicate that the studied samples are groove binders. Electrophoretic methods proved that the studied compounds clearly influence calf thymus Topo I (at 5 muM concentration, except for compounds K107, K111 and K114 which were effective at higher concentrations) and human Topo II (K110 partially inhibited Topo II effects even at 5 muM concentration) activity. |
PubMedSearch : Janockova_2016_Mol.Biosyst_12_2910 |
PubMedID: 27412811 |
Janockova J, Zilecka E, Kasparkova J, Brabec V, Soukup O, Kuca K, Kozurkova M (2016)
Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity
Mol Biosyst
12 :2910
Janockova J, Zilecka E, Kasparkova J, Brabec V, Soukup O, Kuca K, Kozurkova M (2016)
Mol Biosyst
12 :2910