| Title : Cloning and expression of the polyhydroxyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product - Jiang_2004_Biotechnol.Lett_26_1585 |
| Author(s) : Jiang Y , Ye J , Wu H , Zhang H |
| Ref : Biotechnol Lett , 26 :1585 , 2004 |
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Abstract :
A polyhydroxyalkanote (PHA) depolymerase gene ( pha Z) was cloned by PCR from Pseudomonas putida and over-expressed in Escherichia coli as inclusion bodies. Nucleotide sequence analysis predicted an 852 bp open reading frame encoding a protein of 283 amino acids with a predicted molecular weight of 31283 Da. The deduced amino acid sequence had at least 80% homology to the PHA depolymerase from other Pseudomonas strains and consisted a conserved lipase box-like sequence (G-X-S(102)-X-G). The inclusion bodies were refolded and biochemically characterized. The depolymerase activity was optimal at 40 degrees C and pH 8. |
| PubMedSearch : Jiang_2004_Biotechnol.Lett_26_1585 |
| PubMedID: 15604801 |
Jiang Y, Ye J, Wu H, Zhang H (2004)
Cloning and expression of the polyhydroxyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product
Biotechnol Lett
26 :1585
Jiang Y, Ye J, Wu H, Zhang H (2004)
Biotechnol Lett
26 :1585