Jung_2021_J.Biol.Eng_15_9

Reference

Title : An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases - Jung_2021_J.Biol.Eng_15_9
Author(s) : Jung DH , Yong JH , Hwang W , Yoon MY , Yoon SS
Ref : J Biol Eng , 15 :9 , 2021
Abstract :

Short-chain fatty acids, especially butyrate, play beneficial roles in sustaining gastrointestinal health. However, due to limitations associated with direct consumption of butyrate, there has been interest in using prodrugs of butyrate. Tributyrin (TB), a triglyceride composed of three butyrate molecules and a glycerol, is a well-studied precursor of butyrate. We screened a metagenome library consisting of 5760 bacterial artificial chromosome clones, with DNA inserts originating from mouse microbiomes, and identified two clones that efficiently hydrolyse TB into butyrate. Nucleotide sequence analysis indicated that inserts in these two clones are derived from unknown microbes. BLASTp analysis, however, revealed that each insert contains a gene homologous to acetylesterase or esterase genes, from Clostridium spp. and Bacteroides spp., respectively. Predicted structures of these two proteins both contain serine-histidine-aspartate catalytic triad, highly conserved in the family of esterases. Escherichia coli host expressing each of the two candidate genes invariably produced greater amounts of butyrate in the presence of TB. Importantly, administration of TB together with cloned E. coli cells alleviated inflammatory symptoms in a mouse model of acute colitis. Based on these results, we established an efficient on-site and real-time butyrate production system that releases butyrate in a controlled manner inside the intestine.

PubMedSearch : Jung_2021_J.Biol.Eng_15_9
PubMedID: 33676548

Related information

Substrate Tributyrin

Citations formats

Jung DH, Yong JH, Hwang W, Yoon MY, Yoon SS (2021)
An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases
J Biol Eng 15 :9

Jung DH, Yong JH, Hwang W, Yoon MY, Yoon SS (2021)
J Biol Eng 15 :9