| Title : Cloning, expression, purification and preliminary X-ray analysis of EstN2, a novel archaeal alpha\/beta-hydrolase from Candidatus Nitrososphaera gargensis - Kaljunen_2014_Acta.Crystallogr.F.Struct.Biol.Commun_70_1394 |
| Author(s) : Kaljunen H , Chow J , Streit WR , Mueller-Dieckmann J |
| Ref : Acta Crystallographica F Struct Biol Commun , 70 :1394 , 2014 |
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Abstract :
EstN2 is a novel alpha/beta-hydrolase originating from the ammonia-oxidizing thaumarchaeon Candidatus Nitrososphaera gargensis. The genome of the organism was sequenced and genes conferring putative lipolytic activity were amplified and cloned into Escherichia coli as a heterologous host. Through function-based screening, esterase and lipase activity was detected. A recombinant enzyme designated EstN2 was successfully expressed, purified and crystallized. The crystals belonged to space group I2, with one molecule per asymmetric unit, and diffracted X-rays to 1.5 A resolution. |
| PubMedSearch : Kaljunen_2014_Acta.Crystallogr.F.Struct.Biol.Commun_70_1394 |
| PubMedID: 25286947 |
| Gene_locus related to this paper: nitgg-k0im51 |
| Gene_locus | nitgg-k0im51 |
| Structure | 5A62 |
Kaljunen H, Chow J, Streit WR, Mueller-Dieckmann J (2014)
Cloning, expression, purification and preliminary X-ray analysis of EstN2, a novel archaeal alpha\/beta-hydrolase from Candidatus Nitrososphaera gargensis
Acta Crystallographica F Struct Biol Commun
70 :1394
Kaljunen H, Chow J, Streit WR, Mueller-Dieckmann J (2014)
Acta Crystallographica F Struct Biol Commun
70 :1394