Kaschani_2012_Bioorg.Med.Chem_20_597

Reference

Title : Selective inhibition of plant serine hydrolases by agrochemicals revealed by competitive ABPP - Kaschani_2012_Bioorg.Med.Chem_20_597
Author(s) : Kaschani F , Nickel S , Pandey B , Cravatt BF , Kaiser M , van der Hoorn RA
Ref : Bioorganic & Medicinal Chemistry , 20 :597 , 2012
Abstract :

Organophosphate and -phosphonates and their thio derivatives are often used in agroindustry as herbicides and insecticides, but their potential off-targets in the plant are poorly investigated. Here, we use competitive activity-based protein profiling (ABPP) of serine hydrolases (SHs) to detect targets of these agrochemicals and other compounds in Arabidopsis thaliana. Using broad-range and specific probes, and by overexpression of various SHs in planta, we are able to confirm eight SH-compound interactions, including selective inhibition of carboxylesterase CXE12, prolyloligopeptidase, methylesterase MES2 and tripeptidyl peptidase TPP2. These observations can be used for the design of novel probes and selective inhibitors and may help to assess physiological effects of agrochemicals on crop plants.

PubMedSearch : Kaschani_2012_Bioorg.Med.Chem_20_597
PubMedID: 21764588

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Citations formats

Kaschani F, Nickel S, Pandey B, Cravatt BF, Kaiser M, van der Hoorn RA (2012)
Selective inhibition of plant serine hydrolases by agrochemicals revealed by competitive ABPP
Bioorganic & Medicinal Chemistry 20 :597

Kaschani F, Nickel S, Pandey B, Cravatt BF, Kaiser M, van der Hoorn RA (2012)
Bioorganic & Medicinal Chemistry 20 :597