| Title : A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling - Kathayat_2017_Nat.Chem.Biol_13_150 |
| Author(s) : Kathayat RS , Elvira PD , Dickinson BC |
| Ref : Nat Chemical Biology , 13 :150 , 2017 |
|
Abstract :
Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop 'depalmitoylation probes' (DPPs), small-molecule fluorophores, to monitor the endogenous activity levels of 'erasers' of S-palmitoylation, acylprotein thioesterases (APTs). Live-cell analysis with DPPs reveals rapid growth-factor-mediated inhibition of the depalmitoylation activity of APTs, exposing a novel regulatory mechanism of dynamic lipid signaling. |
| PubMedSearch : Kathayat_2017_Nat.Chem.Biol_13_150 |
| PubMedID: 27992880 |
| Substrate | Depalmitoylation-probe-2 |
Kathayat RS, Elvira PD, Dickinson BC (2017)
A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling
Nat Chemical Biology
13 :150
Kathayat RS, Elvira PD, Dickinson BC (2017)
Nat Chemical Biology
13 :150