Khaleghinejad_2016_J.Genet.Eng.Biotechnol_14_83

Reference

Title : Study the effect of F17S mutation on the chimeric Bacillus thermocatenulatus lipase - Khaleghinejad_2016_J.Genet.Eng.Biotechnol_14_83
Author(s) : Khaleghinejad SH , Motalleb G , Karkhane AA , Aminzadeh S , Yakhchali B
Ref : J Genet Eng Biotechnol , 14 :83 , 2016
Abstract :

Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3) are one of the highest value commercial enzymes as they have potential applications in biotechnology for detergents, food, pharmaceuticals, leather, textiles, cosmetics, and paper industries; and are currently receiving considerable attention because of their potential applications in biotechnology. Bacillus thermocatenulatus Lipase 2 (BTL2) is one of the most important research targets, because of its potential industrial applications. In this study, the effect of substitution Phe17 with Ser in mutated BTL2 lipase, which conserved pentapeptide ((112)Ala-His-Ser-Gln-Gly(116)) was replaced with similar sequences ((207)Gly-Glu-Ser-Ala-Gly(211)) of Candida rugosa lipase (CLR) at the nucleophilic elbow region. Docking results confirmed the mutated lipase to be better than the chimeric lipase. So, cloning was conducted, and the mutated and chimeric btl2 genes were expressed in Escherichia coli, and then the enzymes were purified by anion exchange chromatography. The mutation increased lipase lipolytic activity against most of the applied substrates, with the exception of tributyrin when compared with chimeric lipase. Further, the mutated lipase exhibited higher activity than the chimeric lipase at all temperatures. Optimum pH of the mutated lipase was obtained at pH 9.5, which was more than the chimeric one. Enzyme activity of the mutated lipase in the presence of organic solvents, detergents, and metal ions was also improved than the chimeric lipase.

PubMedSearch : Khaleghinejad_2016_J.Genet.Eng.Biotechnol_14_83
PubMedID: 30647601
Gene_locus related to this paper: bactc-lipas

Related information

Gene_locus bactc-lipas

Citations formats

Khaleghinejad SH, Motalleb G, Karkhane AA, Aminzadeh S, Yakhchali B (2016)
Study the effect of F17S mutation on the chimeric Bacillus thermocatenulatus lipase
J Genet Eng Biotechnol 14 :83

Khaleghinejad SH, Motalleb G, Karkhane AA, Aminzadeh S, Yakhchali B (2016)
J Genet Eng Biotechnol 14 :83