| Title : In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis - Koga_2002_J.Biosci.Bioeng_94_84 |
| Author(s) : Koga Y , Kobayashi K , Yang J , Nakano H , Yamane T |
| Ref : J Biosci Bioeng , 94 :84 , 2002 |
|
Abstract :
A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity. |
| PubMedSearch : Koga_2002_J.Biosci.Bioeng_94_84 |
| PubMedID: 16233275 |
Koga Y, Kobayashi K, Yang J, Nakano H, Yamane T (2002)
In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis
J Biosci Bioeng
94 :84
Koga Y, Kobayashi K, Yang J, Nakano H, Yamane T (2002)
J Biosci Bioeng
94 :84