Kong_2023_Eur.J.Endocrinol__

Reference

Title : In-depth Proteomic Signature of Parathyroid Carcinoma - Kong_2023_Eur.J.Endocrinol__
Author(s) : Kong SH , Lee JH , Bae JM , Hong N , Kim H , Park SY , Choi YJ , Lee S , Rhee Y , Kim SW , Han D , Kim JH , Shin CS
Ref : European Journal of Endocrinology , : , 2023
Abstract :

OBJECTIVE: Diagnosing parathyroid carcinoma (PC) is complicated and controversial that early diagnosis and intervention are often difficult. Therefore, we aimed to elucidate the protein signatures of PC through quantitative proteomic analyses to aid in the early and accurate diagnosis of PC. DESIGN: Retrospective cohort study. METHODS: We performed liquid chromatography with tandem mass spectrometry using formalin-fixed paraffin-embedded samples. For the analyses, 23 PC and 15 parathyroid adenoma (PA) tissues were collected from 6 tertiary hospitals in South Korea. RESULTS: The mean age of the patients was 52 years, and 63% were women. Proteomic expression profiling revealed 304 differentially expressed proteins (DEPs) with a cutoff of p < 0.05, and fold change >1.5. Among DEPs, we identified a set of 5 proteins that can discriminate PC from PA: carbonic anhydrase 4 [CA4], alpha/beta hydrolase domain-containing protein 14B [ABHD14B], laminin subunit beta-2 [LAMB2], CD44 antigen [CD44], and alpha-1-acid glycoprotein 1 [ORM1], that exhibited the highest area under the curve of 0.991 in neural network model. The nuclear percentage of CA4 and LAMB2 in immunohistochemistry were significantly lower in PC tissue than in the PA (CA4: 2.77 +/- 1.96%, 26.2 +/- 3.45%, p < 0.001; LAMB2: 6.86 +/- 3.46%, 38.54 +/- 4.13%, p < 0.001). The most enriched canonical pathways in PC included glycoprotein-6 signaling and mammalian target of rapamycin (mTOR). CONCLUSION: We identified key proteins differentially expressed between PC and PA using proteomic analyses of parathyroid neoplasms. These findings may help to diagnose PC accurately and elucidate potential therapeutic targets.

PubMedSearch : Kong_2023_Eur.J.Endocrinol__
PubMedID: 36995894
Gene_locus related to this paper: human-CIB

Related information

Gene_locus human-CIB

Citations formats

Kong SH, Lee JH, Bae JM, Hong N, Kim H, Park SY, Choi YJ, Lee S, Rhee Y, Kim SW, Han D, Kim JH, Shin CS (2023)
In-depth Proteomic Signature of Parathyroid Carcinoma
European Journal of Endocrinology :

Kong SH, Lee JH, Bae JM, Hong N, Kim H, Park SY, Choi YJ, Lee S, Rhee Y, Kim SW, Han D, Kim JH, Shin CS (2023)
European Journal of Endocrinology :