Title : Fenitroxon insensitive acetylcholinesterases of the housefly, Musca domestica associated with point mutations - Kozaki_2001_Insect.Biochem.Mol.Biol_31_991 |
Author(s) : Kozaki T , Shono T , Tomita T , Kono Y |
Ref : Insect Biochemistry & Molecular Biology , 31 :991 , 2001 |
Abstract :
The cDNA of AChE in the housefly, Musca domestica, was sequenced and individual flies were genotyped by this gene in an inhibition assay of AChE activity with an organophaspate, fenitroxon. Mutations at Gly(342) and Tyr(407), which are reportedly conserved in resistant strains of Drosophila, were associated with the insensitivity to fenitroxon. Two other mutations, Ile(162) and Val(260), did not have an apparent effect on insensitivity. However, the four mutations are located in the active site of the enzyme, and therefore the non-neutral mutations in this gene are considered to cause the insensitivity of AChE in the development of insecticide resistance of the housefly. |
PubMedSearch : Kozaki_2001_Insect.Biochem.Mol.Biol_31_991 |
PubMedID: 11483435 |
Gene_locus related to this paper: musdo-ACHE |
Mutation | G262A_musdo-ACHE G262V_musdo-ACHE F327Y_musdo-ACHE V180L_musdo-ACHE I82M_musdo-ACHE |
Gene_locus | musdo-ACHE |
Kozaki T, Shono T, Tomita T, Kono Y (2001)
Fenitroxon insensitive acetylcholinesterases of the housefly, Musca domestica associated with point mutations
Insect Biochemistry & Molecular Biology
31 :991
Kozaki T, Shono T, Tomita T, Kono Y (2001)
Insect Biochemistry & Molecular Biology
31 :991