Title : Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens towards alcohols and carboxylic acids - Krebsfanger_1998_J.Biotechnol_60_105 |
Author(s) : Krebsfanger N , Schierholz K , Bornscheuer UT |
Ref : J Biotechnol , 60 :105 , 1998 |
Abstract :
A recombinant esterase from Pseudomonas fluorescens (PFE) was produced from E. coli cultures and the enantioselectivity towards a series of racemic substrates was investigated. PFE exhibited high rate and enantioselectivity in the acylation of alpha-phenyl ethanol with vinyl acetate in toluene (E > 100) and the hydrolysis of the corresponding acetate in phosphate buffer (E = 58). In sharp contrast, extremely low enantioselectivity (E from 1.1 to 7) was found for the acylation of a series of 1,2-O-protected glycerol derivatives and the hydrolysis of 3-phenylbutyric acid methylester. Almost no reaction occurred with alpha-phenyl propanol and its acetate and 2-phenylbutyric acid ethylester. |
PubMedSearch : Krebsfanger_1998_J.Biotechnol_60_105 |
PubMedID: 9571805 |
Gene_locus related to this paper: psefl-este |
Gene_locus | psefl-este |
Krebsfanger N, Schierholz K, Bornscheuer UT (1998)
Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens towards alcohols and carboxylic acids
J Biotechnol
60 :105
Krebsfanger N, Schierholz K, Bornscheuer UT (1998)
J Biotechnol
60 :105