psefl-este
Pseudomonas fluorescens SIK WI (and strain SBW25) esterase PFEI
Comment
Relationship
Family : Haloperoxidase
Block : X
Position in NCBI Life Tree : Pseudomonas fluorescens
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Gammaproteobacteria N E > Pseudomonadales N E > Pseudomonadaceae N E > Pseudomonas N E > Pseudomonas fluorescens group N E > Pseudomonas fluorescens N EMolecular evidence
No mutation
3T4U - L29I Mutation in an Aryl Esterase from Pseudomonas fluorescens Leads to Unique Peptide Flip and Increased Activity 1
3T52 - L29I Mutation in an Aryl Esterase from Pseudomonas fluorescens Leads to Unique Peptide Flip and Increased Activity 2
3IA2 - Pseudomonas fluorescens esterase complexed to the R-enantiomer of a sulfonate transition state analog
3HI4 - Switching catalysis from hydrolysis to perhydrolysis in P. fluorescens esterase
3HEA - The L29P\/L124I mutation of Pseudomonas fluorescens esterase
1VA4 - Pseudomonas fluorescens aryl esterase
No kinetic
No disease
Sequence
Peptide
MSTFVAKDGT QIYFKDWGSG KPVLFSHGWL LDADMWEYQM EYLSSRGYRT IAFDRRGFGR SDQPWTGNDY DTFADDIAQL IEHLDLKEVT LVGFSMGGGD VARYIARHGS ARVAGLVLLG AVTPLFGQKP DYPQGVPLDV FARFKTELLK DRAQFISDFN APFYGINKGQ VVSQGVQTQT LQIALLASLK ATVDCVTAFA ETDFRPDMAK IDVPTLVIHG DGDQIVPFET TGKVAAELIK GAELKVYKDA PHGFAVTHAQ QLNEDLLAFL KR
References (15)
| Title : AsiteDesign: a Semirational Algorithm for an Automated Enzyme Design - Roda_2023_J.Phys.Chem.B__ |
| Author(s) : Roda S , Terholsen H , Meyer JRH , Canellas-Sole A , Guallar V , Bornscheuer U , Kazemi M |
| Ref : J Phys Chem B , : , 2023 |
| PubMedID: 36944360 |
| Gene_locus related to this paper: psefl-este |
| Title : New structural motif for carboxylic acid perhydrolases - Yin_2013_Chemistry_19_3037 |
| Author(s) : Yin DT , Purpero VM , Fujii R , Jing Q , Kazlauskas RJ |
| Ref : Chemistry , 19 :3037 , 2013 |
| PubMedID: 23325572 |
| Gene_locus related to this paper: psefl-este |
| Title : Promiscuous enantioselective (-)-gamma-lactamase activity in the Pseudomonas fluorescens esterase I - Torres_2012_Org.Biomol.Chem_10_3388 |
| Author(s) : Torres LL , Schliessmann A , Schmidt M , Silva-Martin N , Hermoso JA , Berenguer J , Bornscheuer UT , Hidalgo A |
| Ref : Org Biomol Chem , 10 :3388 , 2012 |
| PubMedID: 22359066 |
| Gene_locus related to this paper: psefl-este |
| Title : Different active-site loop orientation in serine hydrolases versus acyltransferases - Jiang_2011_Chembiochem_12_768 |
| Author(s) : Jiang Y , Morley KL , Schrag JD , Kazlauskas RJ |
| Ref : Chembiochem , 12 :768 , 2011 |
| PubMedID: 21351219 |
| Gene_locus related to this paper: psefl-este |
| Title : Switching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase - Yin_2010_Biochemistry_49_1931 |
| Author(s) : Yin DL , Bernhardt P , Morley KL , Jiang Y , Cheeseman JD , Purpero V , Schrag JD , Kazlauskas RJ |
| Ref : Biochemistry , 49 :1931 , 2010 |
| PubMedID: 20112920 |
| Gene_locus related to this paper: psefl-este |
| Title : Converting an esterase into an epoxide hydrolase - Jochens_2009_Angew.Chem.Int.Ed.Engl_48_3532 |
| Author(s) : Jochens H , Stiba K , Savile C , Fujii R , Yu JG , Gerassenkov T , Kazlauskas RJ , Bornscheuer UT |
| Ref : Angew Chem Int Ed Engl , 48 :3532 , 2009 |
| PubMedID: 19350592 |
| Gene_locus related to this paper: psefl-este |
| Title : Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens - Schliessmann_2009_Chembiochem_10_2920 |
| Author(s) : Schliessmann A , Hidalgo A , Berenguer J , Bornscheuer UT |
| Ref : Chembiochem , 10 :2920 , 2009 |
| PubMedID: 19847842 |
| Gene_locus related to this paper: psefl-este |
| Title : A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution - Hidalgo_2008_Protein.Eng.Des.Sel_21_567 |
| Author(s) : Hidalgo A , Schliessmann A , Molina R , Hermoso J , Bornscheuer UT |
| Ref : Protein Engineering Des Sel , 21 :567 , 2008 |
| PubMedID: 18559369 |
| Gene_locus related to this paper: psefl-este |
| Title : Structure of an aryl esterase from Pseudomonas fluorescens - Cheeseman_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_1237 |
| Author(s) : Cheeseman JD , Tocilj A , Park S , Schrag JD , Kazlauskas RJ |
| Ref : Acta Crystallographica D Biol Crystallogr , 60 :1237 , 2004 |
| PubMedID: 15213385 |
| Gene_locus related to this paper: psefl-este |
| Title : Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate - Horsman_2003_Chemistry_9_1933 |
| Author(s) : Horsman GP , Liu AM , Henke E , Bornscheuer UT , Kazlauskas RJ |
| Ref : Chemistry , 9 :1933 , 2003 |
| PubMedID: 12740839 |
| Gene_locus related to this paper: psefl-este |
| Title : Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay - Henke_1999_Biol.Chem_380_1029 |
| Author(s) : Henke E , Bornscheuer UT |
| Ref : Biol Chem , 380 :1029 , 1999 |
| PubMedID: 10494857 |
| Gene_locus related to this paper: psefl-este |
| Title : Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens towards alcohols and carboxylic acids - Krebsfanger_1998_J.Biotechnol_60_105 |
| Author(s) : Krebsfanger N , Schierholz K , Bornscheuer UT |
| Ref : J Biotechnol , 60 :105 , 1998 |
| PubMedID: 9571805 |
| Gene_locus related to this paper: psefl-este |
| Title : A bacterial esterase is homologous with non-haem haloperoxidases and displays brominating activity - Pelletier_1995_Microbiology_141_459 |
| Author(s) : Pelletier I , Altenbuchner J |
| Ref : Microbiology , 141 :459 , 1995 |
| PubMedID: 7704276 |
| Gene_locus related to this paper: psefl-este |
| Title : Cloning and nucleotide sequence of an esterase gene from Pseudomonas fluorescens and expression of the gene in Escherichia coli - Choi_1990_Agric.Biol.Chem_54_2039 |
| Author(s) : Choi KD , Jeohn GH , Rhee JS , Yoo OJ |
| Ref : Agric and Biological Chemistry , 54 :2039 , 1990 |
| PubMedID: 1368608 |
| Gene_locus related to this paper: psefl-este |