Lack_2008_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_64_2

Reference

Title : Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis - Lack_2008_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_64_2
Author(s) : Lack N , Lowe ED , Liu J , Eltis LD , Noble ME , Sim E , Westwood IM
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 64 :2 , 2008
Abstract :

Tuberculosis is a major cause of death worldwide. Understanding of the pathogenicity of Mycobacterium tuberculosis has been advanced by gene analysis and has led to the identification of genes that are important for intracellular survival in macrophages. One of these genes encodes HsaD, a meta-cleavage product (MCP) hydrolase that catalyzes the hydrolytic cleavage of a carbon-carbon bond in cholesterol metabolism. This paper describes the production of HsaD as a recombinant protein and, following crystallization, the determination of its three-dimensional structure to 2.35 A resolution by X-ray crystallography at the Diamond Light Source in Oxfordshire, England. To the authors' knowledge, this study constitutes the first report of a structure determined at the new synchrotron facility. The volume of the active-site cleft of the HsaD enzyme is more than double the corresponding active-site volumes of related MCP hydrolases involved in the catabolism of aromatic compounds, consistent with the specificity of HsaD for steroids such as cholesterol. Knowledge of the structure of the enzyme facilitates the design of inhibitors.

PubMedSearch : Lack_2008_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_64_2
PubMedID: 18097091
Gene_locus related to this paper: myctu-Rv3569c

Related information

Substrate 4,9-DHSA
Gene_locus 4,9-DHSA    myctu-Rv3569c
Structure 4,9-DHSA    myctu-Rv3569c    2VF2

Citations formats

Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM (2008)
Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis
Acta Crystallographica Sect F Struct Biol Cryst Commun 64 :2

Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM (2008)
Acta Crystallographica Sect F Struct Biol Cryst Commun 64 :2