Lautert_2014_Springerplus_3_506

Reference

Title : Individual in vitro effects of ochratoxin A, deoxynivalenol and zearalenone on oxidative stress and acetylcholinesterase in lymphocytes of broiler chickens - Lautert_2014_Springerplus_3_506
Author(s) : Lautert C , Ferreiro L , Wolkmer P , Paim FC , da Silva CB , Jaques JA , Lopes ST , Santurio JM
Ref : Springerplus , 3 :506 , 2014
Abstract :

The contamination of consumer food and animal feed with toxigenic fungi has resulted in economic losses worldwide in animal industries. Mycotoxins are highly biologically reactive secondary metabolites and can inhibit protein synthesis and cell multiplication. Considering the cytotoxicity of mycotoxins, this experiment was performed to determine the in vitro influence of ochratoxin A, deoxynivalenol and zearalenone on lipid peroxidation in lymphocytes of broiler chickens at different concentrations. This study has also evaluated whether the presence of these mycotoxins changes the acetylcholinesterase activity in lymphocytes, which is involved in the regulation of immune and inflammatory responses. Blood lymphocytes of broiler chickens were isolated through density gradient centrifugation and incubated with the respective mycotoxins at concentrations of 0.001, 0.01, 0.1 and 1 mug/mL. Lipid peroxidation, which was evaluated through the amount of malondialdehyde measured in a thiobarbituric acid-reactive species test, and the enzymatic activity were analyzed at 24, 48 and 72 h. Results of the lipid peroxidation evaluation showed an increasing cytotoxicity relation: ochratoxin A > deoxynivalenol > zearalenone. Conversely, cytotoxicity was valued as zearalenone > deoxynivalenol > ochratoxin A in relation to the acetylcholinesterase enzymatic activity. At a concentration of 1 mug/mL, ochratoxin A and deoxynivalenol induced the highest cellular oxidative stress levels and the highest enzymatic activity at the majority of time points. However, the same mycotoxins, except at 1 mug/mL concentration, induced a reduction of lymphocytic lipid peroxidation 72 h after incubation, suggesting the action of a compensatory mechanism in these cells.

PubMedSearch : Lautert_2014_Springerplus_3_506
PubMedID: 25279298

Related information

Substrate Ochratoxin-A    Zearalenone

Citations formats

Lautert C, Ferreiro L, Wolkmer P, Paim FC, da Silva CB, Jaques JA, Lopes ST, Santurio JM (2014)
Individual in vitro effects of ochratoxin A, deoxynivalenol and zearalenone on oxidative stress and acetylcholinesterase in lymphocytes of broiler chickens
Springerplus 3 :506

Lautert C, Ferreiro L, Wolkmer P, Paim FC, da Silva CB, Jaques JA, Lopes ST, Santurio JM (2014)
Springerplus 3 :506