Leem_2017_Int.J.Biol.Macromol_103_451

Reference

Title : Soluble epoxide hydrolase inhibitory activity of components from Leonurus japonicus - Leem_2017_Int.J.Biol.Macromol_103_451
Author(s) : Leem HH , Lee GY , Lee JS , Lee H , Kim JH , Kim YH
Ref : Int J Biol Macromol , 103 :451 , 2017
Abstract :

One new compound, 10-methoxy-leonurine (1), and four known compounds (2-5) were purified by silica gel, C-18, and Sephadex LH-20 column chromatography from Leonurus japonicus. Their structures were elucidated using one-dimensional (1D)/two-dimensional (2D)-nuclear magnetic resonance (NMR), high-resolution (HR)-electrospray ionization (ESI) mass spectrometry (MS). The compounds were evaluated to determine their inhibition of the catalysis of soluble epoxide hydrolase (sEH). According to the results from in vitro analyses, compounds 1 and 2, which contain guanidine and flavonoid (3), were determined to be potential inhibitors of this enzyme. All compounds were revealed to be non-competitive inhibitors according to Lineweaver-Burk plots. Furthermore, in silico molecular docking indicated that compounds 1-3 are bound to sEH in a similar fashion and have stable binding energies, as calculated by AutoDock 4.2. Molecular dynamics determined the root-mean-square deviation (RMSD), total energy, RMS fluctuation (RMSF), hydrogen bonds, and distance of the complex according to time.

PubMedSearch : Leem_2017_Int.J.Biol.Macromol_103_451
PubMedID: 28501602

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Citations formats

Leem HH, Lee GY, Lee JS, Lee H, Kim JH, Kim YH (2017)
Soluble epoxide hydrolase inhibitory activity of components from Leonurus japonicus
Int J Biol Macromol 103 :451

Leem HH, Lee GY, Lee JS, Lee H, Kim JH, Kim YH (2017)
Int J Biol Macromol 103 :451