Lee JS

References (33)

Title : Changes in dementia treatment patterns associated with changes in the National Policy in South Korea among patients with newly diagnosed Alzheimer's disease between 2011 and 2017: results from the multicenter, retrospective CAPTAIN study - Kim_2024_BMC.Public.Health_24_168
Author(s) : Kim YJ , So KY , Lee HM , Hahn C , Song SH , Lee YS , Kim SW , Park HC , Ryu J , Lee JS , Kang MJ , Kim J , Lee Y , Lee JH
Ref : BMC Public Health , 24 :168 , 2024
Abstract : BACKGROUND: The South Korean government has been actively involved in plans to combat dementia, implementing a series of national strategies and plans since 2008. In July 2014, eligibility for mandatory long-term care insurance (LTCI) was extended to people with dementia enabling access to appropriate long-term care including the cognitive function training program and home nursing service. This study aimed to investigate changes in treatment patterns for Alzheimer's disease (AD) between July 2011 and June 2017 which spanned the 2014 revision. METHODS: This multicenter, retrospective, observational study of patients with newly diagnosed AD analyzed electronic medical records from 17 general hospitals across South Korea. Based on their time of AD diagnosis, subjects were categorized into Cohort 1 (1 July 2011 to 30 June 2014) and Cohort 2 (1 July 2014 to 30 June 2017). RESULTS: Subjects (N=3,997) divided into Cohorts 1 (n=1,998) and 2 (n=1,999), were mostly female (66.4%) with a mean age of 84.4 years. Cohort 1 subjects were significantly older (P<0.0001) and had a lower number of comorbidities (P=0.002) compared with Cohort 2. Mean Mini-Mental State Examination (MMSE) scores in Cohorts 1 and 2 at the time of AD diagnosis or start of initial treatment were 16.9 and 17.1, respectively (P=0.2790). At 1 year, mean MMSE scores in Cohorts 1 and 2 increased to 17.9 and 17.4, respectively (P=0.1524). Donepezil was the most frequently administered medication overall (75.0%), with comparable rates between cohorts. Rates of medication persistence were <=98% for acetylcholinesterase inhibitor or memantine therapy. Discontinuation and switch treatment rates were significantly lower (49.7% vs. 58.0%; P<0.0001), and mean duration of initial treatment significantly longer, in Cohort 2 vs. 1 (349.3 vs. 300.2 days; P<0.0001). CONCLUSIONS: Comparison of cohorts before and after revision of the national LTCI system for dementia patients found no significant difference in mean MMSE scores at the time of AD diagnosis or start of initial treatment. The reduction in the proportion of patients who discontinued or changed their initial treatment, and the significant increase in mean duration of treatment, were observed following revision of the LTCI policy which enabled increased patient access to long-term care.
ESTHER : Kim_2024_BMC.Public.Health_24_168
PubMedSearch : Kim_2024_BMC.Public.Health_24_168
PubMedID: 38216922

Title : Bioinformatic Expansion of Borosins Uncovers Trans-Acting Peptide Backbone N-Methyltransferases in Bacteria - Cho_2022_Biochemistry_61_183
Author(s) : Cho H , Lee H , Hong K , Chung H , Song I , Lee JS , Kim S
Ref : Biochemistry , 61 :183 , 2022
Abstract : Backbone N-methylation is one of the prominent peptide modifications that can greatly enhance the pharmacological properties of a peptide. Naturally occurring backbone N-methylated peptides are produced via nonribosomal or ribosomal pathways, the latter of which was only recently identified in the borosin family of ribosomally synthesized and post-translationally modified peptides. Although previous bioinformatic analyses have revealed new putative genes for borosin biosynthesis, the natural scope of structural and biosynthetic diversity of the borosin family has not been thoroughly explored. Here, we report a comprehensive overview of the borosin family of peptide natural products. Using a genome mining approach, we identified more than 1400 new putative biosynthetic gene clusters for borosins and demonstrated that, unlike those previously reported, most of them are found in bacterial genomes and encode a precursor peptide unfused to its cognate methyltransferase enzyme. Biochemical analysis confirmed the backbone N-methylation of the precursor peptide in trans in eight enzyme-precursor pairs and revealed two novel types of enzyme-recognizing sequences in the precursor peptide. This work significantly expands the biosynthetic diversity of borosins and paves the way for the enzymatic production of diverse backbone N-methylated peptides.
ESTHER : Cho_2022_Biochemistry_61_183
PubMedSearch : Cho_2022_Biochemistry_61_183
PubMedID: 35061348

Title : Neurotoxic effects of different sizes of plastics (nano, micro, and macro) on juvenile common carp (Cyprinus carpio) - Hamed_2022_Front.Mol.Neurosci_15_1028364
Author(s) : Hamed M , Martyniuk CJ , Naguib M , Lee JS , Sayed AEH
Ref : Front Mol Neurosci , 15 :1028364 , 2022
Abstract : Using common carp as a model, we assessed the effects of polyethylene (PE) plastics on the brain. We measured activity of acetylcholinesterase (AChE), monoamine oxidase (MAO), and the content of nitric oxide (NO) in carp brain following exposure to 100 mg/L of either macroplastics (MaP), microplastics (MPs), or nanoplastic (NPs) for 15 days compared to an unexposed group. Following exposure, each biochemical biomarker was reduced 30-40%, with a higher magnitude of change corresponding to the smaller size of the particles (NPs > MPs > MaPs). In the carp tectum, exposure for 15 days to plastic particles caused varying degrees of necrosis, fibrosis, changes in blood capillaries, tissue detachment, edema, degenerated connective tissues, and necrosis in large cerebellar neurons and ganglion cells. In the carp retina, there was evidence for necrosis, degeneration, vacuolation, and curvature in the inner layer. Here we provide evidence that exposure to plastic particles can be associated with neurotoxicity in common carp.
ESTHER : Hamed_2022_Front.Mol.Neurosci_15_1028364
PubMedSearch : Hamed_2022_Front.Mol.Neurosci_15_1028364
PubMedID: 36340695

Title : A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes - Karottki_2021_Metab.Eng_66_114
Author(s) : Karottki KJC , Hefzi H , Li S , Pedersen LE , Spahn PN , Joshi C , Ruckerbauer D , Bort JAH , Thomas A , Lee JS , Borth N , Lee GM , Kildegaard HF , Lewis NE
Ref : Metab Eng , 66 :114 , 2021
Abstract : Media and feed optimization have fueled many-fold improvements in mammalian biopharmaceutical production, but genome editing offers an emerging avenue for further enhancing cell metabolism and bioproduction. However, the complexity of metabolism, involving thousands of genes, makes it unclear which engineering strategies will result in desired traits. Here we present a comprehensive pooled CRISPR screen for CHO cell metabolism, including ~16,000 gRNAs against ~2500 metabolic enzymes and regulators. Using this screen, we identified a glutamine response network in CHO cells. Glutamine is particularly important since it is often over-fed to drive increased TCA cycle flux, but toxic ammonia may accumulate. With the screen we found one orphan glutamine-responsive gene with no clear connection to our network. Knockout of this novel and poorly characterized lipase, Abhd11, substantially increased growth in glutamine-free media by altering the regulation of the TCA cycle. Thus, the screen provides an invaluable targeted platform to comprehensively study genes involved in any metabolic trait, and elucidate novel regulators of metabolism.
ESTHER : Karottki_2021_Metab.Eng_66_114
PubMedSearch : Karottki_2021_Metab.Eng_66_114
PubMedID: 33813034
Gene_locus related to this paper: human-ABHD11

Title : Co-Treatment of Copper Oxide Nanoparticle and Carbofuran Enhances Cardiotoxicity in Zebrafish Embryos - Saputra_2021_Int.J.Mol.Sci_22_
Author(s) : Saputra F , Uapipatanakul B , Lee JS , Hung SM , Huang JC , Pang YC , Munoz JER , Macabeo APG , Chen KH , Hsiao CD
Ref : Int J Mol Sci , 22 : , 2021
Abstract : The use of chemicals to boost food production increases as human consumption also increases. The insectidal, nematicidal and acaricidal chemical carbofuran (CAF), is among the highly toxic carbamate pesticide used today. Alongside, copper oxide nanoparticles (CuO) are also used as pesticides due to their broad-spectrum antimicrobial activity. The overuse of these pesticides may lead to leaching into the aquatic environments and could potentially cause adverse effects to aquatic animals. The aim of this study is to assess the effects of carbofuran and copper oxide nanoparticles into the cardiovascular system of zebrafish and unveil the mechanism behind them. We found that a combination of copper oxide nanoparticle and carbofuran increases cardiac edema in zebrafish larvae and disturbs cardiac rhythm of zebrafish. Furthermore, molecular docking data show that carbofuran inhibits acetylcholinesterase (AChE) activity in silico, thus leading to impair cardiac rhythms. Overall, our data suggest that copper oxide nanoparticle and carbofuran combinations work synergistically to enhance toxicity on the cardiovascular performance of zebrafish larvae.
ESTHER : Saputra_2021_Int.J.Mol.Sci_22_
PubMedSearch : Saputra_2021_Int.J.Mol.Sci_22_
PubMedID: 34361024

Title : Improving the organic solvent resistance of lipase a from Bacillus subtilis in water-ethanol solvent through rational surface engineering - Min_2021_Bioresour.Technol_337_125394
Author(s) : Min K , Kim HT , Park SJ , Lee S , Jung YJ , Lee JS , Yoo YJ , Joo JC
Ref : Bioresour Technol , 337 :125394 , 2021
Abstract : Given that lipase is an enzyme applicable in various industrial fields and water-miscible organic solvents are important reaction media for developing industrial-scale biocatalysis, a structure-based strategy was explored to stabilize lipase A from Bacillus subtilis in a water-ethanol cosolvent. Site-directed mutagenesis of ethanol-interacting sites resulted in 4 mutants, i.e., Ser16Gly, Ala38Gly, Ala38Thr, and Leu108Asn, which were stable in 50% ethanol and had up to 1.8-fold higher stability than the wild-type. In addition, Leu108Asn was more thermostable at 45 degreesC than the wild type. The results discussed in this study not only provide insights into strategies for enzyme engineering to improve organic solvent resistance but also suggest perspectives on pioneering routes for constructing enzyme-based biorefineries to produce value-added fuels and chemicals.
ESTHER : Min_2021_Bioresour.Technol_337_125394
PubMedSearch : Min_2021_Bioresour.Technol_337_125394
PubMedID: 34134054

Title : Neostigmine for Treating Acute Colonic Pseudo-Obstruction in Neurocritically Ill Patients - Kim_2021_J.Clin.Neurol_17_563
Author(s) : Kim TJ , Torres L , Paz A , Lee JS , Park SH , Choi HA , Ko SB
Ref : J Clin Neurol , 17 :563 , 2021
Abstract : BACKGROUND AND PURPOSE: Acute colonic pseudo-obstruction (ACPO) is a common but understudied complication in neurocritically ill patients. The acetylcholinesterase inhibitor neostigmine can be used to treat ACPO in patients who do not respond to conventional treatment. This study investigated the effectiveness and adverse events when using neostigmine to manage ACPO in neurocritically ill patients. METHODS: This retrospective study investigated patients with ACPO who were treated using neostigmine in the neurological intensive-care units at two centers between March 2017 and August 2020. Neostigmine was administered intravenously or subcutaneously (at doses ranging from 0.25 mg to 2 mg) according to the protocols at the two centers. The outcomes were bowel movements and the changes in colon diameters on abdominal radiographs. Safety events such as bradycardia, vomiting, salivation, and sweating were evaluated. RESULTS: This study included 31 subjects with a mean age of 46.8 years (65.4% males). All patients had a bowel movement at a median of 120 minutes after administering neostigmine. The colon diameter decreased by a median of 17.5 mm (paired t-test: p<0.001) regardless of the dose and treatment protocols. Multilevel analysis confirmed that the mean colon diameter decreased from 66 mm pretreatment to 47.5 mm posttreatment (p<0.001), with an intraclass correlation coefficient of 13%. Three patients (9.7%) exhibited hypersalivation, sweating, bradycardia, and vomiting. Bradycardia (heart rate, 42 beats/minute) occurred in one patient (3.2%), and was successfully managed by injecting atropine. CONCLUSIONS: Neostigmine injection is a safe and effective treatment option for ACPO in neurocritically ill patients who fail to respond to conservative management.
ESTHER : Kim_2021_J.Clin.Neurol_17_563
PubMedSearch : Kim_2021_J.Clin.Neurol_17_563
PubMedID: 34595865

Title : Angiopoietin-like protein 3 governs LDL-cholesterol levels through endothelial lipase-dependent VLDL clearance - Adam_2020_J.Lipid.Res_61_1271
Author(s) : Adam RC , Mintah IJ , Alexa-Braun CA , Shihanian LM , Lee JS , Banerjee P , Hamon SC , Kim HI , Cohen JC , Hobbs HH , Van Hout C , Gromada J , Murphy AJ , Yancopoulos GD , Sleeman MW , Gusarova V
Ref : J Lipid Res , 61 :1271 , 2020
Abstract : Angiopoietin-like protein (ANGPTL)3 regulates plasma lipids by inhibiting LPL and endothelial lipase (EL). ANGPTL3 inactivation lowers LDL-C independently of the classical LDLR-mediated pathway and represents a promising therapeutic approach for individuals with homozygous familial hypercholesterolemia due to LDLR mutations. Yet, how ANGPTL3 regulates LDL-C levels is unknown. Here, we demonstrate in hyperlipidemic humans and mice that ANGPTL3 controls VLDL catabolism upstream of LDL. Using kinetic, lipidomic, and biophysical studies, we show that ANGPTL3 inhibition reduces VLDL-lipid content and size, generating remnant particles that are efficiently removed from the circulation. This suggests that ANGPTL3 inhibition lowers LDL-C by limiting LDL particle production. Mechanistically, we discovered that EL is a key mediator of ANGPTL3's novel pathway. Our experiments revealed that, although dispensable in the presence of LDLR, EL-mediated processing of VLDL becomes critical for LDLR-independent particle clearance. In the absence of EL and LDLR, ANGPTL3 inhibition perturbed VLDL catabolism, promoted accumulation of atypical remnants, and failed to reduce LDL-C. Taken together, we uncover ANGPTL3 at the helm of a novel EL-dependent pathway that lowers LDL-C in the absence of LDLR.
ESTHER : Adam_2020_J.Lipid.Res_61_1271
PubMedSearch : Adam_2020_J.Lipid.Res_61_1271
PubMedID: 32646941

Title : pH-Dependent Expression, Stability, and Activity of Malassezia restricta MrLip5 Lipase - Park_2020_Ann.Dermatol_32_473
Author(s) : Park M , Lee JS , Jung WH , Lee YW
Ref : Ann Dermatol , 32 :473 , 2020
Abstract : BACKGROUND: The lipophilic yeasts Malassezia spp. are normally resident on the surface of the human body, and often associated with various skin diseases. Of the 18 known Malassezia spp., Malassezia restricta is the most predominantly identified Malassezia sp. found on the human skin. Malassezia possesses a large number of genes encoding lipases to degrade human sebum triglycerides into fatty acids, which are required not only for their growth, but also trigger skin diseases. Previously, we have shown that MrLIP5 (MRET_0930), one of the 12 lipase genes in the genome of M. restricta, and is the most frequently expressed lipase gene in the scalp of patients with dandruff. OBJECTIVE: In this study, we aimed to analyze the activity, stability, and expression of MrLip5, with particular focus on pH. METHODS: We heterologously expressed MrLip5 in Escherichia coli, and purified and analyzed its activity and expression under different pH conditions. RESULTS: We found that MrLip5 was most active and stable and highly expressed under alkaline conditions, which is similar to that of the diseased skin surface. CONCLUSION: Our results suggest that the activity and expression of MrLip5 are pH-dependent, and that this lipase may play an essential role at the M. restricta-host interface during disease progression.
ESTHER : Park_2020_Ann.Dermatol_32_473
PubMedSearch : Park_2020_Ann.Dermatol_32_473
PubMedID: 33911790

Title : Anti-Obesity Effect of DKB-117 through the Inhibition of Pancreatic Lipase and alpha-Amylase Activity - Kim_2020_Nutrients_12_
Author(s) : Kim DH , Park YH , Lee JS , Jeong HI , Lee KW , Kang TH
Ref : Nutrients , 12 : , 2020
Abstract : This study sought to evaluate the effects of Phaseolus multiflorus var. albus Bailey extract (PM extract) and Pleurotus eryngii var. ferulae extract (PF extract) on the inhibition of digestive enzymes and to confirm the anti-obesity effect of DKB-117 (a mixture of PM extract and PF extract) in digestive enzyme inhibition in a mouse model of obesity induced by a high-fat diet. In in vitro studies, PM extract and PF extract have increased dose-dependent inhibitory activity on alpha-amylase (Inhibitory concentration (IC(50) value: 6.13 mg/mL)) and pancreatic lipase (IC(50) value; 1.68 mg/mL), respectively. High-fat diet-induced obese mice were orally administered DKB-117 extracts at concentrations of 100, 200, and 300 mg/kg/day, while a positive control group was given orlistat (pancreatic lipase inhibitor) and Garcinia cambogia (inhibiting the enzymes needed to synthesize carbohydrates into fat) at concentrations of 40 and 200 mg/kg/day, respectively, for eight weeks. As a result, body weight, fat mass (total fat mass, abdominal fat, and subcutaneous fat) detected with microcomputed tomography, fat mass (abdominal fat and inguinal fat) after an autopsy, and liver triglyceride levels were decreased significantly in the DKB-117 (300 mg/kg/day) group compared to those in the HFD control group. Additionally, we obtained results indicating that the presence of carbohydrates was found more in the DKB-117-300 (300 mg/kg/day) group than in the HFD control group. These data clearly show that DKB-117 extracts are expected to have an anti-obesity effect through a complex mechanism that promotes carbohydrate release through the inhibition of carbohydrate-degrading enzymes while blocking lipid absorption through lipase inhibition.
ESTHER : Kim_2020_Nutrients_12_
PubMedSearch : Kim_2020_Nutrients_12_
PubMedID: 33036193

Title : In vitro and in silico investigation of anthocyanin derivatives as soluble epoxide hydrolase inhibitors - Kim_2018_Int.J.Biol.Macromol_112_961
Author(s) : Kim JH , Cho IS , Ryu J , Lee JS , Kang JS , Kang SY , Kim YH
Ref : Int J Biol Macromol , 112 :961 , 2018
Abstract : Anthocyanin derivatives are well-known secondary constituents contained in fruits. The inhibitory activity of anthocyanin derivatives toward soluble epoxide hydrolase (sEH) was tested for potential applications in the treatment of cardiovascular diseases. Anthocyanin derivatives 1-5 showed dose-dependent inhibitory activity toward sEH, with IC50 values ranging from 4.3+/-0.2 to 25.3+/-2.6muM. Lineweaver-Burk plots showed that all anthocyanin derivatives preferentially interacted with allosteric sites instead of active sites as noncompetitive (1-3) and mixed (4 and 5) inhibitors. Furthermore, the cavity located next to the active site may interact with anthocyanin derivatives (1-5) by molecular docking. Among the tested derivatives, (4) bonded with key amino acids at two loops around the binding site for 10ns. Finally, anthocyanin derivatives (1-5) are potential inhibitors of sEH, and anthocyanin-rich fruits may be useful for the targeted treatment of cardiovascular diseases via sEH inhibition.
ESTHER : Kim_2018_Int.J.Biol.Macromol_112_961
PubMedSearch : Kim_2018_Int.J.Biol.Macromol_112_961
PubMedID: 29447963

Title : Observational Study of Clinical and Functional Progression Based on Initial Brain MRI Characteristics in Patients with Alzheimer's Disease - Choi_2018_J.Alzheimers.Dis_66_1721
Author(s) : Choi H , Yang Y , Han HJ , Jeong JH , Park MY , Kim YB , Jo KD , Choi JY , Kang KH , Kang H , Kwon DY , Yoo BG , Lee HJ , Shin BS , Jeon SM , Kwon OD , Kim JS , Lee SJ , Kim Y , Park TH , Kim YJ , Yang HJ , Park HY , Shin HE , Lee JS , Jung YH , Lee AY , Shin DI , Shin KJ , Park KH
Ref : J Alzheimers Dis , 66 :1721 , 2018
Abstract : BACKGROUND: Magnetic resonance imaging (MRI) is a useful tool to predict the diagnosis and progression of Alzheimer's disease (AD), especially for primary physicians. However, the correlation between baseline MRI findings and AD progression has not been fully established. OBJECTIVE: To investigate the correlation between hippocampal atrophy (HA) and white matter hyperintensities (WMH) on initial brain MRI images and the degree of cognitive decline and functional changes over 1 year. METHODS: In this prospective, 12-month observational study, dementia outpatients were recruited from 29 centers across South Korea. Baseline assessments of HA and WMH on baseline brain MRI were derived as well as cognitive function, dementia severity, activities of daily living, and acetylcholinesterase inhibitor (AChEI) use. Follow-up assessments were conducted at 6 and 12 months. RESULTS: Among 899 enrolled dementia patients, 748 were diagnosed with AD of whom 654 (87%) were taking AChEIs. Baseline WMH showed significant correlations with age, current alcohol consumption, and Clinical Dementia Rating score; baseline HA was correlated with age, family history, physical exercise, and the results of cognitive assessments. Among the AChEI group, changes in the Korean version of the Instrumental Activities of Daily Living (K-IADL) were correlated with the severity of HA on baseline brain MRI, but not with the baseline severity of WMH. In the no AChEI group, changes in K-IADL were correlated with the severity of WMH and HA at baseline. CONCLUSION: Baseline MRI findings could be a useful tool for predicting future clinical outcomes by primary physicians, especially in relation to patients' functional status.
ESTHER : Choi_2018_J.Alzheimers.Dis_66_1721
PubMedSearch : Choi_2018_J.Alzheimers.Dis_66_1721
PubMedID: 30452413

Title : Cholinesterases inhibition studies of biological active compounds from the rhizomes of Alpinia officinarum Hance and in silico molecular dynamics - Lee_2018_Int.J.Biol.Macromol_120_2442
Author(s) : Lee JS , Kim JH , Han YK , Ma JY , Kim YH , Li W , Yang SY
Ref : Int J Biol Macromol , 120 :2442 , 2018
Abstract : Six diarylheptanoids (1-6) and two flavonoids (7 and 8) derived from Alpinia officinarum were evaluated for their ability to inhibit acetylcholinesterase. Compound 1 showed the highest degree of inhibition, with an IC50 of approximately 2muM, followed by moderate degrees of inhibition by 2, 4 and 7, with IC50 values ranging from 20 to 40muM. The remaining isolated compounds 3, 5, 6 and 8 had IC50 values greater than 50muM. Enzyme kinetic studies showed that the compounds with high or moderate activity were competitive inhibitors, anchored to the active site of acetylcholinesterase. In particular, compounds 1 and 2 were docked at slightly different positions from those occupied by 4 and 7. Furthermore, molecular dynamics studies showed that compound 1 maintained its interactions with residues Thr74 and Phe295 throughout the simulation trajectory. Our findings suggest that compound 1 is a potential therapeutically relevant inhibitor of acetylcholinesterase.
ESTHER : Lee_2018_Int.J.Biol.Macromol_120_2442
PubMedSearch : Lee_2018_Int.J.Biol.Macromol_120_2442
PubMedID: 30193916

Title : The genome of the marine medaka Oryzias melastigma - Kim_2018_Mol.Ecol.Resour_18_656
Author(s) : Kim HS , Lee BY , Han J , Jeong CB , Hwang DS , Lee MC , Kang HM , Kim DH , Lee D , Kim J , Choi IY , Lee JS
Ref : Mol Ecol Resour , 18 :656 , 2018
Abstract : Marine medaka (Oryzias melastigma) is considered to be a useful fish model for marine and estuarine ecotoxicology studies and has good potential for field-based population genomics because of its geographical distribution in Asian estuarine and coastal areas. In this study, we present the first whole-genome draft of O. melastigma. The genome assembly consists of 8,602 scaffolds (N50 = 23.737 Mb) and a total genome length of 779.4 Mb. A total of 23,528 genes were predicted, and 12,670 gene families shared with three teleost species (Japanese medaka, mangrove killifish and zebrafish) were identified. Genome analyses revealed that the O. melastigma genome is highly heterozygous and contains a large number of repeat sequences. This assembly represents a useful genomic resource for fish scientists.
ESTHER : Kim_2018_Mol.Ecol.Resour_18_656
PubMedSearch : Kim_2018_Mol.Ecol.Resour_18_656
PubMedID: 29451363
Gene_locus related to this paper: oryme-a0a3b3dpk3 , oryme-a0a3b3c959 , oryme-a0a3b3d3r3 , oryme-a0a3b3d4c8 , oryme-a0a3b3bnt1 , oryme-a0a3b3caa8 , oryme-a0a3b3bl32 , oryme-a0a3b3da95 , oryme-a0a3b3dps7 , oryme-a0a3b3dej7 , oryme-a0a3b3bpw5 , oryme-a0a3b3c014

Title : Deastringent Peel Extracts of Persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) Protect Neuronal PC-12 and SH-SY5Y Cells against Oxidative Stress - Jeong_2018_J.Microbiol.Biotechnol_28_1094
Author(s) : Jeong DW , Cho CH , Lee JS , Lee SH , Kim T , Kim DO
Ref : J Microbiol Biotechnol , 28 :1094 , 2018
Abstract : The peel of astringent persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) is a by-product of dried persimmon (gotgam). We investigated if deastringent peel extracts of persimmon cv. Cheongdo-Bansi had antioxidative and neuroprotective properties. Two different extracts were prepared: thermally and nonthermally treated persimmon peel extracts (TPE and NTPE, respectively). Both TPE and NTPE were fractionated sequentially in n-hexane, chloroform, ethyl acetate, n-butanol, and water. The TPE and NTPE ethyl acetate fractions had the highest total phenolic and flavonoid contents as well as antioxidant capacities among all the fractions. Pretreatment of neuronal PC-12 and SH-SY5Y cells with the TPE and NTPE ethyl acetate fractions increased cell viability after exposure to oxidative stress. The ethyl acetate fraction of TPE attenuated oxidative stress inside both PC-12 and SH-SY5Y cells more effectively than that of NTPE. Furthermore, the TPE and NTPE ethyl acetate fractions inhibited acetylcholinesterase and butyrylcholinesterase. Analysis of ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry results revealed gallic acid, kaempferol, kaempferol-3-O-galactoside, kaempferol-3-O-glucoside, quercetin, quercetin-3-O-galactoside, quercetin-3-O-galactoside-2'-O-gallate, and quercetin-3-O-glucoside as the major phenolics of the TPE and NTPE ethyl acetate fractions. Taken together, these results suggest that the ethyl acetate fraction of deastringent persimmon peel is rich in antioxidants and has potential as a functional food to reduce oxidative stress.
ESTHER : Jeong_2018_J.Microbiol.Biotechnol_28_1094
PubMedSearch : Jeong_2018_J.Microbiol.Biotechnol_28_1094
PubMedID: 29975999

Title : LIPG signaling promotes tumor initiation and metastasis of human basal-like triple-negative breast cancer - Lo_2018_Elife_7_e31334
Author(s) : Lo PK , Yao Y , Lee JS , Zhang Y , Huang W , Kane MA , Zhou Q
Ref : Elife , 7 : , 2018
Abstract : Current understanding of aggressive human basal-like triple-negative breast cancer (TNBC) remains incomplete. In this study, we show endothelial lipase (LIPG) is aberrantly overexpressed in basal-like TNBCs. We demonstrate that LIPG is required for in vivo tumorigenicity and metastasis of TNBC cells. LIPG possesses a lipase-dependent function that supports cancer cell proliferation and a lipase-independent function that promotes invasiveness, stemness and basal/epithelial-mesenchymal transition features of TNBC. Mechanistically, LIPG executes its oncogenic function through its involvement in interferon-related DTX3L-ISG15 signaling, which regulates protein function and stability by ISGylation. We show that DTX3L, an E3-ubiquitin ligase, is required for maintaining LIPG protein levels in TNBC cells by inhibiting proteasome-mediated LIPG degradation. Inactivation of LIPG impairs DTX3L-ISG15 signaling, indicating the existence of DTX3L-LIPG-ISG15 signaling. We further reveal LIPG-ISG15 signaling is lipase-independent. We demonstrate that DTX3L-LIPG-ISG15 signaling is essential for malignancies of TNBC cells. Targeting this pathway provides a novel strategy for basal-like TNBC therapy.
ESTHER : Lo_2018_Elife_7_e31334
PubMedSearch : Lo_2018_Elife_7_e31334
PubMedID: 29350614
Gene_locus related to this paper: human-LIPG

Title : Soluble epoxide hydrolase inhibitory activity of components from Leonurus japonicus - Leem_2017_Int.J.Biol.Macromol_103_451
Author(s) : Leem HH , Lee GY , Lee JS , Lee H , Kim JH , Kim YH
Ref : Int J Biol Macromol , 103 :451 , 2017
Abstract : One new compound, 10-methoxy-leonurine (1), and four known compounds (2-5) were purified by silica gel, C-18, and Sephadex LH-20 column chromatography from Leonurus japonicus. Their structures were elucidated using one-dimensional (1D)/two-dimensional (2D)-nuclear magnetic resonance (NMR), high-resolution (HR)-electrospray ionization (ESI) mass spectrometry (MS). The compounds were evaluated to determine their inhibition of the catalysis of soluble epoxide hydrolase (sEH). According to the results from in vitro analyses, compounds 1 and 2, which contain guanidine and flavonoid (3), were determined to be potential inhibitors of this enzyme. All compounds were revealed to be non-competitive inhibitors according to Lineweaver-Burk plots. Furthermore, in silico molecular docking indicated that compounds 1-3 are bound to sEH in a similar fashion and have stable binding energies, as calculated by AutoDock 4.2. Molecular dynamics determined the root-mean-square deviation (RMSD), total energy, RMS fluctuation (RMSF), hydrogen bonds, and distance of the complex according to time.
ESTHER : Leem_2017_Int.J.Biol.Macromol_103_451
PubMedSearch : Leem_2017_Int.J.Biol.Macromol_103_451
PubMedID: 28501602

Title : Pseudomonas aeruginosa sabotages the generation of host proresolving lipid mediators - Flitter_2017_Proc.Natl.Acad.Sci.U.S.A_114_136
Author(s) : Flitter BA , Hvorecny KL , Ono E , Eddens T , Yang J , Kwak DH , Bahl CD , Hampton TH , Morisseau C , Hammock BD , Liu X , Lee JS , Kolls JK , Levy BD , Madden DR , Bomberger JM
Ref : Proc Natl Acad Sci U S A , 114 :136 , 2017
Abstract : Recurrent Pseudomonas aeruginosa infections coupled with robust, damaging neutrophilic inflammation characterize the chronic lung disease cystic fibrosis (CF). The proresolving lipid mediator, 15-epi lipoxin A4 (15-epi LXA4), plays a critical role in limiting neutrophil activation and tissue inflammation, thus promoting the return to tissue homeostasis. Here, we show that a secreted P. aeruginosa epoxide hydrolase, cystic fibrosis transmembrane conductance regulator inhibitory factor (Cif), can disrupt 15-epi LXA4 transcellular biosynthesis and function. In the airway, 15-epi LXA4 production is stimulated by the epithelial-derived eicosanoid 14,15-epoxyeicosatrienoic acid (14,15-EET). Cif sabotages the production of 15-epi LXA4 by rapidly hydrolyzing 14,15-EET into its cognate diol, eliminating a proresolving signal that potently suppresses IL-8-driven neutrophil transepithelial migration in vitro. Retrospective analyses of samples from patients with CF supported the translational relevance of these preclinical findings. Elevated levels of Cif in bronchoalveolar lavage fluid were correlated with lower levels of 15-epi LXA4, increased IL-8 concentrations, and impaired lung function. Together, these findings provide structural, biochemical, and immunological evidence that the bacterial epoxide hydrolase Cif disrupts resolution pathways during bacterial lung infections. The data also suggest that Cif contributes to sustained pulmonary inflammation and associated loss of lung function in patients with CF.
ESTHER : Flitter_2017_Proc.Natl.Acad.Sci.U.S.A_114_136
PubMedSearch : Flitter_2017_Proc.Natl.Acad.Sci.U.S.A_114_136
PubMedID: 27980032
Gene_locus related to this paper: pseae-PA2934

Title : Gongjin-Dan Enhances Hippocampal Memory in a Mouse Model of Scopolamine-Induced Amnesia - Lee_2016_PLoS.One_11_e0159823
Author(s) : Lee JS , Hong SS , Kim HG , Lee HW , Kim WY , Lee SK , Son CG
Ref : PLoS ONE , 11 :e0159823 , 2016
Abstract : We evaluated the neuropharmacological effects of Gongjin-Dan (GJD) on the memory impairment caused by scopolamine injection. BALB/c mice were orally treated with GJD (100, 200, or 400 mg/kg, daily) or tacrine (THA, 10 mg/kg) for 10 days, and scopolamine (2 mg/kg) was injected intraperitoneally. The radial arm maze and passive avoidance tests were performed to evaluate the animal's learning and memory. Scopolamine increased the task completing time, the number of total errors (reference and working memory error) in the radial arm maze task, and the latency time in the passive avoidance test, which were significantly ameliorated by treatment with GJD. The GJD treatment also attenuated the scopolamine-induced hyperactivation of acetylcholinesterase activity, and suppression of the expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and their receptors in the hippocampus. These effects of GJD were supported by both the doublecortin (DCX)-positive staining and Nissl staining, which were used to measure hippocampal neurogenesis and atrophy, respectively. These findings strongly suggest that GJD exerts a potent anti-amnesic effect, and its underlying mechanism might involve the modulation of cholinergic activity.
ESTHER : Lee_2016_PLoS.One_11_e0159823
PubMedSearch : Lee_2016_PLoS.One_11_e0159823
PubMedID: 27483466

Title : Production of Biodiesel from Acid Oil via a Two-Step Enzymatic Transesterification - Choi_2016_J.Oleo.Sci_65_913
Author(s) : Choi N , Lee JS , Kwak J , Lee J , Kim IH
Ref : J Oleo Sci , 65 :913 , 2016
Abstract : A two-step enzymatic transesterification process in a solvent-free system has been developed as a novel approach to the production of biodiesel using acid oil from rice bran oil soapstock. The acid oil consisted of 53.7 wt% fatty acids, 2.4 wt% monoacylglycerols, 9.1 wt% diacylglycerols, 28.8 wt% triacylglycerols, and 6.0 wt% others. Three immobilized lipases were evaluated as potential biocatalysts, including Novozym 435 from Candida antarctica, Lipozyme RM IM from Rhizomucor miehei, and Lipozyme TL IM from Thermomyces lanuginosus. The effects of molar ratio of acid oil to ethanol, temperature, and enzyme loading were investigated to determine the optimum conditions for the transesterification with the three immobilized lipases. The optimum conditions of the three immobilized lipases were a molar ratio of 1:5 (acid oil to ethanol), the temperature range of 30-40 degC, and the enzyme loading range of 5-10%. The two-step transesterification was then conducted under the optimum conditions of each lipase. The stepwise use of Novozym 435 and Lipozyme TL IM or Lipozyme RM IM and Lipozyme TL IM resulted in similar or higher levels of yield to the individual lipases. The maximum yields obtained in both stepwise uses were ca. 92%.
ESTHER : Choi_2016_J.Oleo.Sci_65_913
PubMedSearch : Choi_2016_J.Oleo.Sci_65_913
PubMedID: 27733740

Title : Kinetics and molecular docking studies of cholinesterase inhibitors derived from water layer of Lycopodiella cernua (L.) Pic. Serm. (II) - Hung_2015_Chem.Biol.Interact_240_74
Author(s) : Hung TM , Lee JS , Chuong NN , Kim JA , Oh SH , Woo MH , Choi JS , Min BS
Ref : Chemico-Biological Interactions , 240 :74 , 2015
Abstract : Acetylcholinesterase (AChE) inhibitors increase the availability of acetylcholine in central cholinergic synapses and are the most promising drugs currently available for the treatment of Alzheimer's disease (AD). Our screening study indicated that the water fraction of the methanolic extract of Lycopodiella cernua (L.) Pic. Serm. significantly inhibited AChE in vitro. Bioassay-guided fractionation led to the isolation of a new lignan glycoside, lycocernuaside A (12), and fourteen known compounds (1-11 and 13-15). Compound 7 exhibited the most potent AChE inhibitory activity with an IC50 value of 0.23 muM. Compound 15 had the most potent inhibitory activity against BChE and BACE1 with IC50 values of 0.62 and 2.16 muM, respectively. Compounds 4 and 7 showed mixed- and competitive-type AChE inhibition. Compound 7 noncompetitively inhibited BChE whereas 15 showed competitive and 8, 13, and 14 showed mixed-type inhibition. The docking results for complexes with AChE or BChE revealed that inhibitors 4, 7, and 15 stably positioned themselves in several pocket/catalytic domains of the AChE and BChE residues.
ESTHER : Hung_2015_Chem.Biol.Interact_240_74
PubMedSearch : Hung_2015_Chem.Biol.Interact_240_74
PubMedID: 26297990

Title : Hippocampal memory enhancing activity of pine needle extract against scopolamine-induced amnesia in a mouse model - Lee_2015_Sci.Rep_5_9651
Author(s) : Lee JS , Kim HG , Lee HW , Han JM , Lee SK , Kim DW , Saravanakumar A , Son CG
Ref : Sci Rep , 5 :9651 , 2015
Abstract : We evaluated the neuropharmacological effects of 30% ethanolic pine needle extract (PNE) on memory impairment caused by scopolamine injection in mice hippocampus. Mice were orally pretreated with PNE (25, 50, and 100 mg/kg) or tacrine (10 mg/kg) for 7 days, and scopolamine (2 mg/kg) was injected intraperitoneally, 30 min before the Morris water maze task on first day. To evaluate memory function, the Morris water maze task was performed for 5 days consecutively. Scopolamine increased the escape latency and cumulative path-length but decreases the time spent in target quadrant, which were ameliorated by pretreatment with PNE. Oxidant-antioxidant balance, acetylcholinesterase activity, neurogenesis and their connecting pathway were abnormally altered by scopolamine in hippocampus and/or sera, while those alterations were recovered by pretreatment with PNE. As lipid peroxidation, 4HNE-positive stained cells were ameliorated in hippocampus pretreated with PNE. Pretreatment with PNE increased the proliferating cells and immature neurons against hippocampal neurogenesis suppressed by scopolamine, which was confirmed by ki67- and DCX-positive stained cells. The expression of brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element-binding protein (pCREB) in both protein and gene were facilitated by PNE pretreatment. These findings suggest that PNE could be a potent neuropharmacological drug against amnesia, and its possible mechanism might be modulating cholinergic activity via CREB-BDNF pathway.
ESTHER : Lee_2015_Sci.Rep_5_9651
PubMedSearch : Lee_2015_Sci.Rep_5_9651
PubMedID: 25974329

Title : Inhibitory effects of biocides on transcription and protein activity of acetylcholinesterase in the intertidal copepod Tigriopus japonicus - Lee_2014_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_167C_147
Author(s) : Lee JW , Kim BM , Jeong CB , Won EJ , Rhee JS , Lee JS
Ref : Comparative Biochemistry & Physiology C Pharmacol Toxicol , 167C :147 , 2014
Abstract : Acetlycholinesterase (AChE) is a serine esterase that plays an important role in the hydrolytic degradation of acetylcholine. We investigated the modulatory potential of T. japonicus-AChE (TJ-AChE) for biocide response by cloning, sequencing, and characterizing the full-length genomic DNA of the TJ-AChE1 and TJ-AChE2 genes. The deduced TJ-AChE proteins were highly conserved across species and were distinctively separated into two subtypes, AChE1 and AChE2. Each TJ-AChE protein was closely phylogenetically clustered with invertebrate AChE1 and AChE2 proteins. Transcriptional level of TJ-AChE1 was higher than TJ-AChE2 in all developmental stages. TJ-AChE1 mRNA decreased in response to five biocides (alachlor, chlorpyrifos, dimethoate, endosulfan, lindane,) but not in the molinate-exposed group. TJ-AChE2 decreased significantly only in response to chlorpyrifos and lindane. TJ-AChE enzymatic activity was significantly inhibited when exposed to alachlor, chlorpyrifos, endosulfan, or lindane for 24h. This study elucidates potential endogenous mechanisms of biocide-induced neurotoxicity in T. japonicus.
ESTHER : Lee_2014_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_167C_147
PubMedSearch : Lee_2014_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_167C_147
PubMedID: 25468639
Gene_locus related to this paper: tigja-ACHE1 , tigja-ACHE2

Title : Ramlibacter solisilvae sp. nov., isolated from forest soil, and emended description of the genus Ramlibacter - Lee_2014_Int.J.Syst.Evol.Microbiol_64_1317
Author(s) : Lee HJ , Lee SH , Lee SS , Lee JS , Kim Y , Kim SC , Jeon CO
Ref : Int J Syst Evol Microbiol , 64 :1317 , 2014
Abstract : A Gram-staining-negative, strictly aerobic, white-colony-forming bacterium, designated strain 5-10(T), was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods or coccoids, showing oxidase- and catalase-positive reactions. Growth was observed at 10-37 degrees C (optimum, 30 degrees C), at pH 5.0-9.0 (optimum, pH 7.0) and in the presence of 0-1.0 % (w/v) NaCl (optimum, 0-0.5 %). The major cellular fatty acids were summed feature 3 (comprising C16 : 1omega6c and/or C16 : 1omega7c), C16 : 0, C10 : 0 3-OH and summed feature 8 (comprising C18 : 1omega6c and/or C18 : 1omega7c). The G+C content of the genomic DNA was 69.9 mol% and the only respiratory quinone detected was ubiquinone 8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5-10(T) formed a tight phyletic lineage with members of the genus Ramlibacter. Strain 5-10(T) was most closely related to Ramlibacter tataouinensis TTB310(T) (97.3 %), but the DNA-DNA relatedness level between the two strains was 38.2+/-1.8 %. Based on phenotypic, chemotaxonomic and molecular features, strain 5-10(T) was shown to represent a novel species of the genus Ramlibacter, for which the name Ramlibacter solisilvae sp. nov. is proposed. The type strain is 5-10(T) ( = KACC 17567(T) = JCM 19319(T)). An emended description of the genus Ramlibacter is also proposed.
ESTHER : Lee_2014_Int.J.Syst.Evol.Microbiol_64_1317
PubMedSearch : Lee_2014_Int.J.Syst.Evol.Microbiol_64_1317
PubMedID: 24425747
Gene_locus related to this paper: 9burk-a0a127juv8

Title : Ethanol extract of Astragali Radix and Salviae Miltiorrhizae Radix, Myelophil, exerts anti-amnesic effect in a mouse model of scopolamine-induced memory deficits - Lee_2014_J.Ethnopharmacol_153_782
Author(s) : Lee JS , Kim HG , Han JM , Kim DW , Yi MH , Son SW , Kim YA , Choi MK , Son CG
Ref : J Ethnopharmacol , 153 :782 , 2014
Abstract : ETHNOPHARMACOLOGICAL RELEVANCE: Myelophil, a combination of extracts taken from Astragali Radix and Salviae Miltiorrhizae Radix, is a traditional Chinese medicine used for the treatment of chronic fatigue-associated disorders. Here we examined the ability of Myelophil to alleviate memory impairment in a mouse model. We aimed to investigate whether Myelophil has the pharmacological effects on memory deficits associated with brain dysfunctions using an animal model. MATERIALS AND
METHODS: Ten week-old male C57BL/6N mice were pretreated with Myelophil (50, 100, or 200 mg/kg), or tacrine (10 mg/kg) for 7 days, and then intraperitoneally injected with scopolamine (1 mg/kg). Memory-related behaviors were evaluated using the Morris water maze for 5 days. Levels of biomarkers of oxidative stress, antioxidant activity, acetylcholinesterase (AChE) activity, and extracellular signal-regulated kinase (ERK) were measured in brain tissues.
RESULTS: Scopolamine treatment increased the escape latency time and shortened time spent in the target quadrant; these effects were ameliorated by pretreatment with Myelophil. Scopolamine-induced changes in reactive oxygen species (ROS), malondialehyde (MDA), and AChE activity were significantly attenuated in mice pretreated with Myelophil. Recovery of antioxidant capacities, including total glutathione (GSH) content, and the activities of GSH-reductase, GSH-S-transferase, and catalase was also evident in Myelophil-treated mice. The strongest effects were seen for ERK and muscarinic acetylcholine receptor 1 (mAChR1) at both the protein and gene expression levels, with significant amelioration of expression levels in the Myelophil pretreatment group.
CONCLUSIONS: These results suggest that Myelophil confers anti-amnesic properties in a mouse model of memory impairment, driven in part by the modulation of cholinergic activity.
ESTHER : Lee_2014_J.Ethnopharmacol_153_782
PubMedSearch : Lee_2014_J.Ethnopharmacol_153_782
PubMedID: 24690775

Title : Superpriming of synaptic vesicles after their recruitment to the readily releasable pool - Lee_2013_Proc.Natl.Acad.Sci.U.S.A_110_15079
Author(s) : Lee JS , Ho WK , Neher E , Lee SH
Ref : Proc Natl Acad Sci U S A , 110 :15079 , 2013
Abstract : Recruitment of release-competent vesicles during sustained synaptic activity is one of the major factors governing short-term plasticity. During bursts of synaptic activity, vesicles are recruited to a fast-releasing pool from a reluctant vesicle pool through an actin-dependent mechanism. We now show that newly recruited vesicles in the fast-releasing pool do not respond at full speed to a strong Ca(2+) stimulus, but require approximately 4 s to mature to a "superprimed" state. Superpriming was found to be altered by agents that modulate the function of unc13 homolog proteins (Munc13s), but not by calmodulin inhibitors or actin-disrupting agents. These findings indicate that recruitment and superpriming of vesicles are regulated by separate mechanisms, which require integrity of the cytoskeleton and activation of Munc13s, respectively. We propose that refilling of the fast-releasing vesicle pool proceeds in two steps, rapid actin-dependent "positional priming," which brings vesicles closer to Ca(2+) sources, followed by slower superpriming, which enhances the Ca(2+) sensitivity of primed vesicles.
ESTHER : Lee_2013_Proc.Natl.Acad.Sci.U.S.A_110_15079
PubMedSearch : Lee_2013_Proc.Natl.Acad.Sci.U.S.A_110_15079
PubMedID: 23980146

Title : Effect of pharmaceuticals exposure on acetylcholinesterase (AchE) activity and on the expression of AchE gene in the monogonont rotifer, Brachionus koreanus - Rhee_2013_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_158_216
Author(s) : Rhee JS , Kim BM , Jeong CB , Park HG , Leung KM , Lee YM , Lee JS
Ref : Comparative Biochemistry & Physiology C Pharmacol Toxicol , 158 :216 , 2013
Abstract : Pharmaceuticals are widely used in human and veterinary medicine. However, they are emerging as a significant contaminant in aquatic environments through wastewater. Due to the persistent and accumulated properties of pharmaceuticals via the food web, their potential harmful effects on aquatic animals are a great concern. In this study, we investigated the effects of six pharmaceuticals: acetaminophen, ATP; atenolol, ATN; carbamazepine, CBZ; oxytetracycline, OTC; sulfamethoxazole, SMX; and trimethoprim, TMP on acetylcholinesterase (AChE; EC activity and its transcript expression with chlorpyrifos (as a positive control) in the monogonont rotifer, Brachionus koreanus. ATP, CBZ, and TMP exposure also remarkably inhibited Bk-AChE activity at 100mug/L (24h) and 1000mug/L (12h and 24h). ATP, CBZ, and TMP exposure showed a significant decrease in the Bk-AChE mRNA level in a concentration-dependent manner. However, in the case of OTC and SMX, a slight decrease in Bk-AChE mRNA expression was found but only at the highest concentration. The time-course experiments showed that ATP positively induced Bk-AChE mRNA 12h after exposure at both 100 and 1000mug/L, while the Bk-AChE mRNA expression was significantly downregulated over 6 to 24h after exposure to 1000mug/L of CBZ, OTC, SMX, and TMP. Our findings suggest that Bk-AChE would be a useful biomarker for risk assessment of pharmaceutical compounds as an early signal of their toxicity in aquatic environments. Particularly, ATP, CBZ, and TMP may have a toxic cholinergic effect on rotifer B. koreanus by inhibiting AChE activity.
ESTHER : Rhee_2013_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_158_216
PubMedSearch : Rhee_2013_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_158_216
PubMedID: 24028855

Title : Identification of differentially expressed genes and quantitative expression of complement genes in the liver of marine medaka Oryzias melastigma challenged with Vibrio parahaemolyticus - Bo_2012_Comp.Biochem.Physiol.Part.D.Genomics.Proteomics_7_191
Author(s) : Bo J , Giesy JP , Ye R , Wang KJ , Lee JS , Au DW
Ref : Comparative Biochemistry & Physiology Part D Genomics Proteomics , 7 :191 , 2012
Abstract : The innate immune system of fish is the primary defense against acute diseases. The marine medaka Oryzias melastigma has been shown to be a potential marine fish model for ecotoxicology, but little is known about the innate immune system of this small fish. In this study, suppression subtractive hybridization (SSH) was used to identify differentially expressed immune genes in the liver of O. melastigma infected with Vibrio parahaemolyticus. Among the 396 genes identified, based on NCBI BLAST search of the 1279 sequenced clones in the SSH libraries, 38 (9.6%) were involved in the immune process. Besides, genes involved in biological regulations (5.6%); cellular metabolism (24.7%); general response to stimuli (4.8%); cellular component organization (2.3%); signal transduction (2.5%) and transport process (2.8%) were also obtained. Ten complement component genes involved in four activation pathways were quantified (using q-PCR) and exhibited different patterns of transcription between the control and challenged individuals. The results reported upon here support the feasibility of developing O. melastigma as a marine model fish to understand the basic biological processes related to immune function and for immunotoxicological research. Findings of this study established a genetic platform for studying immune function using O. melastigma.
ESTHER : Bo_2012_Comp.Biochem.Physiol.Part.D.Genomics.Proteomics_7_191
PubMedSearch : Bo_2012_Comp.Biochem.Physiol.Part.D.Genomics.Proteomics_7_191
PubMedID: 22445008

Title : Cholinesterase inhibitors from Cleistocalyx operculatus buds - Min_2010_Arch.Pharm.Res_33_1665
Author(s) : Min BS , Cuong TD , Lee JS , Shin BS , Woo MH , Hung TM
Ref : Arch Pharm Res , 33 :1665 , 2010
Abstract : Five flavonoids, myricetin-3'-methylether 3-O-beta-D: -galactopyranoside (1), myricetin-3',5'-dimethylether 3-O-beta-D: -galactopyranoside (2), quercetin (3), kaempferol (4), and tamarixetin (5) were isolated from the buds of Cleistocalyx operculatus (Myrtaceae). The chemical structures of these compounds were determined on the basis of spectroscopic analyses, including 2D NMR. Their anti-Alzheimer effects were evaluated via acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity assays. All five compounds 1-5 showed potential inhibitory activities against AChE with IC(50) values of 19.9, 37.8, 25.9, 30.4 and 22.3 muM, respectively, while compounds 1, 3, 4 and 5 also possessed BChE inhibitory activity with IC(50) values of 152.5, 177.8, 62.5, and 160.6 muM, respectively.
ESTHER : Min_2010_Arch.Pharm.Res_33_1665
PubMedSearch : Min_2010_Arch.Pharm.Res_33_1665
PubMedID: 21052942

Title : Complete genome sequence of Leuconostoc citreum KM20 - Kim_2008_J.Bacteriol_190_3093
Author(s) : Kim JF , Jeong H , Lee JS , Choi SH , Ha M , Hur CG , Kim JS , Lee S , Park HS , Park YH , Oh TK
Ref : Journal of Bacteriology , 190 :3093 , 2008
Abstract : Leuconostoc citreum is one of the most prevalent lactic acid bacteria during the manufacturing process of kimchi, the best-known Korean traditional dish. We have determined the complete genome sequence of L. citreum KM20. It consists of a 1.80-Mb chromosome and four circular plasmids and reveals genes likely involved in kimchi fermentation and its probiotic effects.
ESTHER : Kim_2008_J.Bacteriol_190_3093
PubMedSearch : Kim_2008_J.Bacteriol_190_3093
PubMedID: 18281406
Gene_locus related to this paper: leuck-b1mwg5 , leuck-b1mwm3 , leuck-b1mxa8 , leuck-b1mxk9 , leuck-b1my46 , leuck-b1mz26 , leuck-b1mwr2

Title : The complete mitochondrial genome of the Korean soft-shelled turtle Pelodiscus sinensis (Testudines, Trionychidae) - Jungt_2006_DNA.Seq_17_471
Author(s) : Jungt SO , Lee YM , Kartavtsev Y , Park IS , Kim DS , Lee JS
Ref : DNA Sequence , 17 :471 , 2006
Abstract : We isolated Korean soft-shelled turtle, Pelodiscus sinensis, mitochondrial DNA by long-polymerase chain reaction (long-PCR) with conserved primers and sequenced this mitochondrial genome (mitogenome) with primer walking using flanking sequences. The P. sinensis mitochondrial DNA has 17,042 bp and its structural organization is conserved compared to those of other reptiles and mammals. To unveil the phylogenetic relationship of the turtles, we used the NJ, MP, and ML analysis methods after inferring those sequences from the mitochondrial 16S rRNA gene. We also compared two P. sinensis variants from Korea and China using the mitochondrial genome. In this study, we report the basic characteristics of the P. sinensis mitochondrial genome, including structural organization and base composition of the rRNAs, tRNAs and protein-coding genes, as well as characteristics of tRNAs. These features are applicable for the study of phylogenetic relationships in turtles.
ESTHER : Jungt_2006_DNA.Seq_17_471
PubMedSearch : Jungt_2006_DNA.Seq_17_471
PubMedID: 17381049
Gene_locus related to this paper: pelsi-k7g2a9 , pelsi-k7f735 , pelsi-k7fcz0 , pelsi-k7fkb6 , pelsi-k7fks6 , pelsi-k7g6q6 , pelsi-k7f9p9 , pelsi-k7fhg2 , pelsi-k7fcu8 , pelsi-k7fst0 , pelsi-k7gc94 , pelsi-k7gh41 , pelsi-k7fmq1 , pelsi-k7fa08 , pelsi-k7f9k7 , pelsi-k7f0f0 , pelsi-k7fma0 , pelsi-k7fn69 , pelsi-k7gjb0 , pelsi-k7ghp3

Title : The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4 - Seo_2005_Nat.Biotechnol_23_63
Author(s) : Seo JS , Chong H , Park HS , Yoon KO , Jung C , Kim JJ , Hong JH , Kim H , Kim JH , Kil JI , Park CJ , Oh HM , Lee JS , Jin SJ , Um HW , Lee HJ , Oh SJ , Kim JY , Kang HL , Lee SY , Lee KJ , Kang HS
Ref : Nat Biotechnol , 23 :63 , 2005
Abstract : We report the complete genome sequence of Zymomonas mobilis ZM4 (ATCC31821), an ethanologenic microorganism of interest for the production of fuel ethanol. The genome consists of 2,056,416 base pairs forming a circular chromosome with 1,998 open reading frames (ORFs) and three ribosomal RNA transcription units. The genome lacks recognizable genes for 6-phosphofructokinase, an essential enzyme in the Embden-Meyerhof-Parnas pathway, and for two enzymes in the tricarboxylic acid cycle, the 2-oxoglutarate dehydrogenase complex and malate dehydrogenase, so glucose can be metabolized only by the Entner-Doudoroff pathway. Whole genome microarrays were used for genomic comparisons with the Z. mobilis type strain ZM1 (ATCC10988) revealing that 54 ORFs predicted to encode for transport and secretory proteins, transcriptional regulators and oxidoreductase in the ZM4 strain were absent from ZM1. Most of these ORFs were also found to be actively transcribed in association with ethanol production by ZM4.
ESTHER : Seo_2005_Nat.Biotechnol_23_63
PubMedSearch : Seo_2005_Nat.Biotechnol_23_63
PubMedID: 15592456
Gene_locus related to this paper: zymmo-DLH , zymmo-GAA , zymmo-metx , zymmo-q5nkz4 , zymmo-q5nmh0 , zymmo-q5nmm8 , zymmo-q5nmn0 , zymmo-q5nmz5 , zymmo-q5nnu4 , zymmo-q5npe2 , zymmo-q5nph2 , zymmo-q5npn6 , zymmo-q5nq91 , zymmo-q5nrh7 , zymmo-q5nnr5

Title : Synthesis of tetrakis(multifluoro-4-pyridyl)porphin derivatives as acetylcholinesterase inhibitors - Moon_2000_Bioorg.Med.Chem.Lett_10_1435
Author(s) : Moon SC , Shin JH , Jeong BH , Kim HS , Yu BS , Lee JS , Lee BS , Namgoong SK
Ref : Bioorganic & Medicinal Chemistry Lett , 10 :1435 , 2000
Abstract : New tetrakis(multifluoro-4-pyridyl)porphin derivatives (2-4) and water soluble porphyrin (5) were synthesized to investigate their interactions with acetylcholinesterase from electric eel. These compounds have been found to be the potent reversible inhibitors of the enzyme with Ki values of microM range. In addition, porphyrin (5) showed broad spectrum of anticancer activities.
ESTHER : Moon_2000_Bioorg.Med.Chem.Lett_10_1435
PubMedSearch : Moon_2000_Bioorg.Med.Chem.Lett_10_1435
PubMedID: 10888326