Title : Expression of a cDNA encoding the glycolipid-anchored form of rat acetylcholinesterase - Legay_1993_FEBS.Lett_315_163 |
Author(s) : Legay C , Bon S , Massoulie J |
Ref : FEBS Letters , 315 :163 , 1993 |
Abstract :
We amplified by PCR and characterized a fragment of cDNA from rat spleen, encoding the distinctive C-terminal region of the acetylcholinesterase (AChE) H subunit. A recombinant vector encoding this subunit was constructed and expressed in COS cells: the H subunits produced glycophosphatidylinositol (GPI)-anchored dimers, showing that the spleen cDNA fragment contained a functional GPI cleavage/attachment site. Using PCR, we did not detect mRNAs encoding AChE H in rat muscle or hypothalamus. In the liver of 16-day rat embryos, we found both H and T transcripts, in agreement with the presence of both GPI-anchored dimers and amphiphilic monomers of type II. In addition, we detected 'read-through' (R) transcripts, in which regular introns are spliced, but the intervening sequence between the common exon 4 and the alternative exon 5 (H) is maintained. |
PubMedSearch : Legay_1993_FEBS.Lett_315_163 |
PubMedID: 8417973 |
Legay C, Bon S, Massoulie J (1993)
Expression of a cDNA encoding the glycolipid-anchored form of rat acetylcholinesterase
FEBS Letters
315 :163
Legay C, Bon S, Massoulie J (1993)
FEBS Letters
315 :163