Leimann_2023_Food.Res.Int_173_113295

Reference

Title : Evaluation of berberine nanoparticles as a strategy to modulate acetylcholinesterase activity - Leimann_2023_Food.Res.Int_173_113295
Author(s) : Leimann FV , de Souza LB , de Oliveira BPM , Rossi BF , da Silva PS , Shiraishi CSH , Kaplum V , Abreu RM , Pereira C , Barros L , Peron AP , Ineu RP , Oechsler BF , Sayer C , de Araujo PHH , Goncalves OH
Ref : Food Res Int , 173 :113295 , 2023
Abstract :

Researchers have concentrated efforts in the search for natural-based reversible inhibitors for cholinesterase enzymes as they may play a key role in the treatment of degenerative diseases. Diverse plant alkaloids can inhibit the action of acetylcholinesterase and, among them, berberine is a promising bioactive. However, berberine has poor water solubility and low bioavailability, which makes it difficult to use in treatment. The solid dispersion technique can improve the water affinity of hydrophobic substances, but berberine solid dispersions have not been extensively studied. Safety testing is also essential to ensure that the berberine-loaded solid dispersions are safe for use. This study investigated the effectiveness of berberine-loaded solid dispersions (SD) as inhibitors of acetylcholinesterase enzyme (AChE). Docking simulation was used to investigate the influence of berberine on AChE, and in vitro assays were conducted to confirm the enzymatic kinetics of AChE in the presence of berberine. Berberine SD also showed improved cytotoxic effects on tumoral cells when dispersed in aqueous media. In vivo assays using Allium cepa were implemented, and no cytotoxicity/genotoxicity was found for the berberine solid dispersion. These results suggest that berberine SD could be a significant step towards safe nanostructures for use in the treatment of neurodegenerative diseases.

PubMedSearch : Leimann_2023_Food.Res.Int_173_113295
PubMedID: 37803607

Related information

Citations formats

Leimann FV, de Souza LB, de Oliveira BPM, Rossi BF, da Silva PS, Shiraishi CSH, Kaplum V, Abreu RM, Pereira C, Barros L, Peron AP, Ineu RP, Oechsler BF, Sayer C, de Araujo PHH, Goncalves OH (2023)
Evaluation of berberine nanoparticles as a strategy to modulate acetylcholinesterase activity
Food Res Int 173 :113295

Leimann FV, de Souza LB, de Oliveira BPM, Rossi BF, da Silva PS, Shiraishi CSH, Kaplum V, Abreu RM, Pereira C, Barros L, Peron AP, Ineu RP, Oechsler BF, Sayer C, de Araujo PHH, Goncalves OH (2023)
Food Res Int 173 :113295