| Title : Actions of two highly potent organophosphorus neuropathy target esterase inhibitors in mammalian cell lines - Li_1997_Toxicol.Lett_92_123 |
| Author(s) : Li W , Casida JE |
| Ref : Toxicol Lett , 92 :123 , 1997 |
|
Abstract :
Neuropathy target esterase (NTE) is inhibited by many organophosphorus compounds that induce delayed neuropathy. This study examines two of the most potent NTE inhibitors, 2-octyl-4H-1,3,2-benzodioxaphosphorin 2-oxide (OBDPO) and ethyl octylphosphonofluoridate (EOPF), in cell lines with neural properties (PC-12 and NB41A3) and of nonneural origin (C6 and HeLa). NTE-like esteratic activity is higher in PC-12, HeLa and C6 cells than in NB41A3 cells and in each case is inhibited 50% by OBDPO and EOPF at 0.03-3.4 nM in vitro and by OBDPO at 0.080-36 nM in situ in culture. An NTE-like protein(s) of about 155 kDa is phosphorylated and labeled by [3H-octyl]OBDPO in these cell lines in the same order as their relative NTE esteratic activity. Cytotoxic levels of OBDPO and EOPF (300-500 microM) are generally 10(5) to > 10(7)-fold higher than required for NTE inhibition. PC-12 cells and OBDPO/[3H]OBDPO and EOPF are therefore suitable for research on non-lethal biochemical disruptions from NTE phosphorylation and aging. |
| PubMedSearch : Li_1997_Toxicol.Lett_92_123 |
| PubMedID: 9295235 |
Li W, Casida JE (1997)
Actions of two highly potent organophosphorus neuropathy target esterase inhibitors in mammalian cell lines
Toxicol Lett
92 :123
Li W, Casida JE (1997)
Toxicol Lett
92 :123