Title : Simple determination of huperzine A in human plasma by liquid chromatographic-tandem mass spectrometric method - Li_2007_Biomed.Chromatogr_21_15 |
Author(s) : Li YX , Jiang XH , Lan K , Wang L |
Ref : Biomedical Chromatography , 21 :15 , 2007 |
Abstract :
Huperzine A is a potent, reversible acetylcholinesterase inhibitor. In the present work, a rapid and sensitive LC-MS-MS method for the determination of huperzine A in human plasma using codeine phosphate as internal standard has been developed and validated. The analyte and internal standard were extracted from plasma using ethyl acetate, chromatographed on a C(18) column (5 microm, 150 x 4.6 mm i.d.) with a mobile phase consisting of 1% formic acid-methanol (40:60, v/v), and detected using a tandem mass spectrometer with a TurboIonSpray ionization interface. The run time was only 2 min. Good linearity was achieved in the range 0.126 -25.2 ng/mL and the limit of detection in plasma was 0.064 ng/mL. The average recovery for huperzine A was 83.4% from plasma. The analytical sensitivity and accuracy of this assay is adequate for characterization of huperzine A in human plasma. |
PubMedSearch : Li_2007_Biomed.Chromatogr_21_15 |
PubMedID: 17080497 |
Li YX, Jiang XH, Lan K, Wang L (2007)
Simple determination of huperzine A in human plasma by liquid chromatographic-tandem mass spectrometric method
Biomedical Chromatography
21 :15
Li YX, Jiang XH, Lan K, Wang L (2007)
Biomedical Chromatography
21 :15