| Title : A Novel Process for the Synthesis of Highly Pure n-3 Polyunsaturated Fatty Acid (PUFA)-Enriched Triglycerides by Combined Transesterification and Ethanolysis - Li_2016_J.Agric.Food.Chem_64_6533 |
| Author(s) : Li D , Wang W , Qin X , Li X , Yang B , Wang Y |
| Ref : Journal of Agricultural and Food Chemistry , 64 :6533 , 2016 |
| Abstract : In this study, a novel two-step enzymatic reaction was developed for the synthesis of highly pure triacylglycerols (TAGs) with a high content of n-3 polyunsaturated fatty acids (PUFAs). Glyceride mixtures were primarily synthesized by Novozym 435-catalyzed transesterification of glycerol and DHA/EPA-rich ethyl esters (EEs), followed by removal of partial glycerides, for the first time, by immobilized mono- and diacylglycerol lipase SMG1-F278N-catalyzed ethanolysis. TAG yield as high as 98.66% was achieved under the optimized conditions, and highly pure (98.75%) n-3 PUFA-enriched TAGs with 88.44% of n-3 PUFA was obtained after molecular distillation at lower temperature (140 degreesC). In addition, the EEs produced during ethanolysis had a FA composition similar to that of the original EEs, making them feasible for cyclic utilization. This was the first study reporting removal of partial glycerides by ethanolysis. Through ethanolysis, a higher purity product could be easily obtained at a relatively low temperature compared with the conventional high-temperature molecular distillation. |
| ESTHER : Li_2016_J.Agric.Food.Chem_64_6533 |
| PubMedSearch : Li_2016_J.Agric.Food.Chem_64_6533 |
| PubMedID: 27540752 |
| Gene_locus related to this paper: malgo-a8puy1 |
| Gene_locus related to this paper: malgo-a8puy1 |
Li D, Wang W, Qin X, Li X, Yang B, Wang Y (2016)
A Novel Process for the Synthesis of Highly Pure n-3 Polyunsaturated Fatty Acid (PUFA)-Enriched Triglycerides by Combined Transesterification and Ethanolysis
Journal of Agricultural and Food Chemistry
64 :6533
Li D, Wang W, Qin X, Li X, Yang B, Wang Y (2016)
Journal of Agricultural and Food Chemistry
64 :6533