Li_2021_LWT_151_112118

Edible grade rice bran oil (RBO) rich in ?-oryzanol and phytosterol but without glycidyl esters (GEs) and monochloropropanediol esters (MCPDEs) was obtained from rice bran by moderate biorefining. High-acid RBO (acid value of 51.22mg KOH/g) was firstly extracted from rice bran and was then moderately biorefined by combining improved enzymatic degumming, dewaxing, enzymatic deacidification, bleaching, and low-temperature purification (120C). Through monitoring the refining process, results showed that the improved enzymatic degumming by combining partial glyceride lipase SMG1-F278N and phospholipase A1 enabled the phosphorus content to quickly decrease to 4.56mg/kg after optimization by response surface methodology. After biorefining, the final RBO with the acid and peroxide value of 0.10mg KOH/g and 2.05mmol/kg reached the grade-one standard of edible oil. Additionally, the final RBO contained 20.66mg/kg ?-oryzanol and 20.63mg/kg phytosterol with the retention rate of 71.67% and 59.2% but with undetectable GEs and MCPDEs. The environmental friendliness of the novel moderate biorefining process and superior product quality of the final product point toward a promising viable process for RBO refining in the further.