| Title : Crystallization and preliminary X-ray diffraction data for the carboxylesterase Est30 from Bacillus stearothermophilus - Liu_2003_Acta.Crystallogr.D.Biol.Crystallogr_59_1472 |
| Author(s) : Liu P , Wang YF , Ewis HE , Abdelal A , Lu CD , Weber IT |
| Ref : Acta Crystallographica D Biol Crystallogr , 59 :1472 , 2003 |
|
Abstract :
Crystals have been grown of the carboxylesterase Est30 from Bacillus stearothermophilus by hanging-drop vapor diffusion using ammonium sulfate as precipitant. The crystals diffracted to better than 2.0 A resolution. X-ray diffraction data were reduced in space group C222(1), with unit-cell parameters a = 55.83, b = 58.15, c = 179.65 A. R(merge) was 0.038 for 17 449 independent reflections with a completeness of 85.1%. V(M) was calculated to be 2.43 A(3) Da(-1), which suggested that there was one molecule of Est30 in the asymmetric unit. These crystals are suitable for structure determination. |
| PubMedSearch : Liu_2003_Acta.Crystallogr.D.Biol.Crystallogr_59_1472 |
| PubMedID: 12876355 |
| Gene_locus related to this paper: geost-est30 |
| Gene_locus | geost-est30 |
Liu P, Wang YF, Ewis HE, Abdelal A, Lu CD, Weber IT (2003)
Crystallization and preliminary X-ray diffraction data for the carboxylesterase Est30 from Bacillus stearothermophilus
Acta Crystallographica D Biol Crystallogr
59 :1472
Liu P, Wang YF, Ewis HE, Abdelal A, Lu CD, Weber IT (2003)
Acta Crystallographica D Biol Crystallogr
59 :1472