geost-est30

Geobacillus stearothermophilus thermostable carboxylesterase Est30

Comment

Engineered protein, Est30 variant KLS-MHETase M14 (I171K, M127S, and G130L)active against MHET

Relationship

Family : CarbLipBact_1

Block : X

Position in NCBI Life Tree : Geobacillus stearothermophilus

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Firmicutes N E > Bacilli N E > Bacillales N E > Bacillaceae N E > Geobacillus N E > Geobacillus stearothermophilus N E

Database

Sequence

Peptide

MMKIVPPKPF FFEAGERAVL LLHGFTGNSA DVRMLGRFLE SKGYTCHAPI YKGHGVPPEE LVHTGPDDWW QDVMNGYEFL KNKGYEKIAV AGLSLGGVFS LKLGYTVPIE GIVTMCAPMY IKSEETMYEG VLEYAREYKK REGKSEEQIE QEMEKFKQTP MKTLKALQEL IADVRDHLDL IYAPTFVVQA RHDEMINPDS ANIIYNEIES PVKQIKWYEQ SGHVITLDQE KDQLHEDIYA FLESLDW

References (5)

Title : Computational design of highly efficient thermostable MHET hydrolases and dual enzyme system for PET recycling - Zhang_2023_Commun.Biol_6_1135
Author(s) : Zhang J , Wang H , Luo Z , Yang Z , Zhang Z , Wang P , Li M , Zhang Y , Feng Y , Lu D , Zhu Y
Ref : Commun Biol , 6 :1135 , 2023
PubMedID: 37945666
Gene_locus related to this paper: geost-est30

Title : Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus - Ewis_2004_Gene_329_187
Author(s) : Ewis HE , Abdelal AT , Lu CD
Ref : Gene , 329 :187 , 2004
PubMedID: 15033540
Gene_locus related to this paper: geost-est30 , geost-est50

Title : Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30 - Liu_2004_J.Mol.Biol_342_551
Author(s) : Liu P , Wang YF , Ewis HE , Abdelal AT , Lu CD , Harrison RW , Weber IT
Ref : Journal of Molecular Biology , 342 :551 , 2004
PubMedID: 15327954
Gene_locus related to this paper: geost-est30

Title : Crystallization and preliminary X-ray diffraction data for the carboxylesterase Est30 from Bacillus stearothermophilus - Liu_2003_Acta.Crystallogr.D.Biol.Crystallogr_59_1472
Author(s) : Liu P , Wang YF , Ewis HE , Abdelal A , Lu CD , Weber IT
Ref : Acta Crystallographica D Biol Crystallogr , 59 :1472 , 2003
PubMedID: 12876355
Gene_locus related to this paper: geost-est30

Title : Molecular cloning and structure of the gene for esterase from a thermophilic bacterium, Bacillus stearothermophilus IFO 12550 -
Author(s) : Kugimiya W , Otani Y , Hashimoto Y
Ref : Biosci Biotechnol Biochem , 56 :2074 , 1992
PubMedID: 1369099
Gene_locus related to this paper: geost-est30