geost-est30
Geobacillus stearothermophilus thermostable carboxylesterase Est30
Comment
Engineered protein, Est30 variant KLS-MHETase M14 (I171K, M127S, and G130L)active against MHET
Relationship
Family : CarbLipBact_1
Block : X
Position in NCBI Life Tree : Geobacillus stearothermophilus
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Firmicutes N E > Bacilli N E > Bacillales N E > Bacillaceae N E > Geobacillus N E > Geobacillus stearothermophilus N EMolecular evidence
Sequence
Peptide
MMKIVPPKPF FFEAGERAVL LLHGFTGNSA DVRMLGRFLE SKGYTCHAPI YKGHGVPPEE LVHTGPDDWW QDVMNGYEFL KNKGYEKIAV AGLSLGGVFS LKLGYTVPIE GIVTMCAPMY IKSEETMYEG VLEYAREYKK REGKSEEQIE QEMEKFKQTP MKTLKALQEL IADVRDHLDL IYAPTFVVQA RHDEMINPDS ANIIYNEIES PVKQIKWYEQ SGHVITLDQE KDQLHEDIYA FLESLDW
References (5)
| Title : Computational design of highly efficient thermostable MHET hydrolases and dual enzyme system for PET recycling - Zhang_2023_Commun.Biol_6_1135 |
| Author(s) : Zhang J , Wang H , Luo Z , Yang Z , Zhang Z , Wang P , Li M , Zhang Y , Feng Y , Lu D , Zhu Y |
| Ref : Commun Biol , 6 :1135 , 2023 |
| Abstract : |
| PubMedSearch : Zhang_2023_Commun.Biol_6_1135 |
| PubMedID: 37945666 |
| Gene_locus related to this paper: geost-est30 |
| Title : Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus - Ewis_2004_Gene_329_187 |
| Author(s) : Ewis HE , Abdelal AT , Lu CD |
| Ref : Gene , 329 :187 , 2004 |
| Abstract : |
| PubMedSearch : Ewis_2004_Gene_329_187 |
| PubMedID: 15033540 |
| Gene_locus related to this paper: geost-est30 , geost-est50 |
| Title : Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30 - Liu_2004_J.Mol.Biol_342_551 |
| Author(s) : Liu P , Wang YF , Ewis HE , Abdelal AT , Lu CD , Harrison RW , Weber IT |
| Ref : Journal of Molecular Biology , 342 :551 , 2004 |
| Abstract : |
| PubMedSearch : Liu_2004_J.Mol.Biol_342_551 |
| PubMedID: 15327954 |
| Gene_locus related to this paper: geost-est30 |
| Title : Crystallization and preliminary X-ray diffraction data for the carboxylesterase Est30 from Bacillus stearothermophilus - Liu_2003_Acta.Crystallogr.D.Biol.Crystallogr_59_1472 |
| Author(s) : Liu P , Wang YF , Ewis HE , Abdelal A , Lu CD , Weber IT |
| Ref : Acta Crystallographica D Biol Crystallogr , 59 :1472 , 2003 |
| Abstract : |
| PubMedSearch : Liu_2003_Acta.Crystallogr.D.Biol.Crystallogr_59_1472 |
| PubMedID: 12876355 |
| Gene_locus related to this paper: geost-est30 |
| Title : Molecular cloning and structure of the gene for esterase from a thermophilic bacterium, Bacillus stearothermophilus IFO 12550 - |
| Author(s) : Kugimiya W , Otani Y , Hashimoto Y |
| Ref : Biosci Biotechnol Biochem , 56 :2074 , 1992 |
| PubMedID: 1369099 |
| Gene_locus related to this paper: geost-est30 |