| Title : Preparation and application of monoclonal antibody against hNDRG2 - Liu_2009_Appl.Biochem.Biotechnol_152_306 |
| Author(s) : Liu X , Hu X , Zhang J , Wang L , Zhang W , Li F , Zhang Y , Yao L |
| Ref : Appl Biochem Biotechnol , 152 :306 , 2009 |
| Abstract : The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5alpha as a 6x His-tagged protein. The purified 6x His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry. |
| ESTHER : Liu_2009_Appl.Biochem.Biotechnol_152_306 |
| PubMedSearch : Liu_2009_Appl.Biochem.Biotechnol_152_306 |
| PubMedID: 18758694 |
Liu X, Hu X, Zhang J, Wang L, Zhang W, Li F, Zhang Y, Yao L (2009)
Preparation and application of monoclonal antibody against hNDRG2
Appl Biochem Biotechnol
152 :306
Liu X, Hu X, Zhang J, Wang L, Zhang W, Li F, Zhang Y, Yao L (2009)
Appl Biochem Biotechnol
152 :306