Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672

Reference

Title : AgHalo: A Facile Fluorogenic Sensor to Detect Drug-Induced Proteome Stress - Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672
Author(s) : Liu Y , Fares M , Dunham NP , Gao Z , Miao K , Jiang X , Bollinger SS , Boal AK , Zhang X
Ref : Angew Chem Int Ed Engl , 56 :8672 , 2017
Abstract : Drug-induced proteome stress that involves protein aggregation may cause adverse effects and undermine the safety profile of a drug. Safety of drugs is regularly evaluated using cytotoxicity assays that measure cell death. However, these assays provide limited insights into the presence of proteome stress in live cells. A fluorogenic protein sensor is reported to detect drug-induced proteome stress prior to cell death. An aggregation prone Halo-tag mutant (AgHalo) was evolved to sense proteome stress through its aggregation. Detection of such conformational changes was enabled by a fluorogenic ligand that fluoresces upon AgHalo forming soluble aggregates. Using 5 common anticancer drugs, we exemplified detection of differential proteome stress before any cell death was observed. Thus, this sensor can be used to evaluate drug safety in a regime that the current cytotoxicity assays cannot cover and be generally applied to detect proteome stress induced by other toxins.
ESTHER : Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672
PubMedSearch : Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672
PubMedID: 28557281
Gene_locus related to this paper: rhoso-halo1

Related information

Gene_locus related to this paper: rhoso-halo1

Citations formats

Liu Y, Fares M, Dunham NP, Gao Z, Miao K, Jiang X, Bollinger SS, Boal AK, Zhang X (2017)
AgHalo: A Facile Fluorogenic Sensor to Detect Drug-Induced Proteome Stress
Angew Chem Int Ed Engl 56 :8672

Liu Y, Fares M, Dunham NP, Gao Z, Miao K, Jiang X, Bollinger SS, Boal AK, Zhang X (2017)
Angew Chem Int Ed Engl 56 :8672