Llorent-Martinez_2017_Front.Pharmacol_8_83

Reference

Title : Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L. czeczottianus and L. nissolia - Llorent-Martinez_2017_Front.Pharmacol_8_83
Author(s) : Llorent-Martinez EJ , Zengin G , Fernandez-de Cordova ML , Bender O , Atalay A , Ceylan R , Mollica A , Mocan A , Uysal S , Guler GO , Aktumsek A
Ref : Front Pharmacol , 8 :83 , 2017
Abstract :

Members of the genus Lathyrus are used as food and as traditional medicines. In order to find new sources of biologically-active compounds, chemical and biological profiles of two Lathyrus species (L. czeczottianus and L. nissolia) were investigated. Chemical profiles were evaluated by HPLC-ESI-MSn, as well as by their total phenolic and flavonoid contents. In addition, antioxidant, enzyme inhibitory, and cytotoxic effects were also investigated. Antioxidant properties were tested by using different assays (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation). Cholinesterases (AChE and BChE), tyrosinase, alpha-amylase, and alpha-glucosidase were used to evaluate enzyme inhibitory effects. Moreover, vitexin (apigenin-8-C-glucoside) and 5-O-caffeoylquinic acid were further subjected to molecular docking experiments to provide insights about their interactions at molecular level with the tested enzymes. In vitro cytotoxic effects were examined against human embryonic kidney cells (HEK293) by using iCELLigence real time cell analysis system. Generally, L. czeczottianus exhibited stronger antioxidant properties than L. nissolia. However, L. nissolia had remarkable enzyme inhibitory effects against cholinesterase, amylase and glucosidase. HPLC-ESI-MSn analysis revealed that flavonoids were major components in these extracts. On the basis of these results, Lathyrus extracts were rich in biologically active components; thus, these species could be utilized to design new phytopharmaceutical and nutraceutical formulations.

PubMedSearch : Llorent-Martinez_2017_Front.Pharmacol_8_83
PubMedID: 28289386

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Llorent-Martinez EJ, Zengin G, Fernandez-de Cordova ML, Bender O, Atalay A, Ceylan R, Mollica A, Mocan A, Uysal S, Guler GO, Aktumsek A (2017)
Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L. czeczottianus and L. nissolia
Front Pharmacol 8 :83

Llorent-Martinez EJ, Zengin G, Fernandez-de Cordova ML, Bender O, Atalay A, Ceylan R, Mollica A, Mocan A, Uysal S, Guler GO, Aktumsek A (2017)
Front Pharmacol 8 :83