Lo Verde_2022_Food.Res.Int_162_111996

Reference

Title : A highly active esterase from Lactobacillus helveticus hydrolyzes chlorogenic acid in sunflower meal to prevent chlorogenic acid induced greening in sunflower protein isolates - Lo Verde_2022_Food.Res.Int_162_111996
Author(s) : Lo Verde C , Pepra-Ameyaw NB , Drucker CT , Okumura TLS , Lyon KA , Muniz JC , Sermet CS , Were Senger L , Owens CP
Ref : Food Res Int , 162 :111996 , 2022
Abstract : Chlorogenic acid (CGA) is an ester between caffeic and quinic acid. It is found in many foods and reacts with free amino groups in proteins at alkaline pH, leading to the formation of an undesirable green pigment in sunflower seed-derived ingredients. This paper presents the biochemical characterization and application of a highly active chlorogenic acid esterase from Lactobacillus helveticus. The enzyme is one of the most active CGA esterases known to date with a K(m) of 0.090smM and a k(cat) of 82.1ss(-1). The CGA esterase is easily expressed recombinantly in E. coli in large yields and is stable over a wide range of pH and temperatures. We characterized CGA esterase's kinetic properties in sunflower meal and demonstrated that the enzyme completely hydrolyzes CGA in the meal. Finally, we showed that CGA esterase treatment of sunflower seed meal enables the production of pale brown sunflower protein isolates using alkaline extraction. This work will allow for more widespread use of sunflower-derived products in applications where neutrally-colored food products are desired.
ESTHER : Lo Verde_2022_Food.Res.Int_162_111996
PubMedSearch : Lo Verde_2022_Food.Res.Int_162_111996
PubMedID: 36461298
Gene_locus related to this paper: lache-u6f2k7

Related information

Gene_locus related to this paper: lache-u6f2k7

Citations formats

Lo Verde C, Pepra-Ameyaw NB, Drucker CT, Okumura TLS, Lyon KA, Muniz JC, Sermet CS, Were Senger L, Owens CP (2022)
A highly active esterase from Lactobacillus helveticus hydrolyzes chlorogenic acid in sunflower meal to prevent chlorogenic acid induced greening in sunflower protein isolates
Food Res Int 162 :111996

Lo Verde C, Pepra-Ameyaw NB, Drucker CT, Okumura TLS, Lyon KA, Muniz JC, Sermet CS, Were Senger L, Owens CP (2022)
Food Res Int 162 :111996