Longhi_1997_Protein.Sci_6_275

Reference

Title : Crystal structure of cutinase covalently inhibited by a triglyceride analogue - Longhi_1997_Protein.Sci_6_275
Author(s) : Longhi S , Mannesse M , Verheij HM , De Haas GH , Egmond M , Knoops-Mouthuy E , Cambillau C
Ref : Protein Science , 6 :275 , 1997
Abstract :

Cutinase from Fusarium solani is a lipolytic enzyme that hydrolyses triglycerides efficiently All the inhibited forms of lipolytic enzymes described so far are based on the use of small organophosphate and organophosphonate inhibitors which bear little resemblance to a natural triglyceride substrate In this article we describe the crystal structure of cutinase covalently inhibited by R)-1,2-dibutyl carbamoylglycero-3-O-p-nitrophenylbutyl-phos phonate a triglyceride analogue mimicking the first tetrahedral intermediate along the reaction pathway The structure which has been solved at 2.3 A reveals that in both the protein molecules of the asymmetric unit the inhibitor is almost completely embedded in the active site crevice The overall shape of the inhibitor is that of a fork the two dibutyl carbamoyl chains point towards the surface of the protein whereas the butyl chain bound to the phosphorous atom is roughly perpendicular to the sn-1 and sn-2 chains The sn-3 chain is accommodated in a rather small pocket at the bottom of the active site crevice thus providing a structural explanation for the preference of cutinase for short acyl chain substrates

PubMedSearch : Longhi_1997_Protein.Sci_6_275
PubMedID: 9041628
Gene_locus related to this paper: fusso-cutas

Related information

Inhibitor BCP
Gene_locus BCP    fusso-cutas
Family BCP    fusso-cutas    Cutinase
Structure BCP    fusso-cutas    Cutinase    1AGY    1CEX    1FFA    1FFB    1FFC    1FFD    1FFE    1OXM

Citations formats

Longhi S, Mannesse M, Verheij HM, De Haas GH, Egmond M, Knoops-Mouthuy E, Cambillau C (1997)
Crystal structure of cutinase covalently inhibited by a triglyceride analogue
Protein Science 6 :275

Longhi S, Mannesse M, Verheij HM, De Haas GH, Egmond M, Knoops-Mouthuy E, Cambillau C (1997)
Protein Science 6 :275