Lu_2024_J.Agric.Food.Chem__

Reference

Title : Efficient Expression and Application of a Modified Rhizomucor miehei Lipase for Simultaneous Production of Biodiesel and Eicosapentaenoic Acid Ethyl Ester from Nannochloropsis Oil - Lu_2024_J.Agric.Food.Chem__
Author(s) : Lu T , Qian Y , Zhu Y , Ju X , Dai W , Xu Q , Yang Q , Li S , Yuan B , Huang J
Ref : Journal of Agricultural and Food Chemistry , : , 2024
Abstract :

Few reports exist on one-step enzymatic methods for the simultaneous production of biodiesel and eicosapentaenoic acid ethyl ester (EPA-EE), a high-value pharmaceutical compound. This study aimed to efficiently express Rhizomucor miehei lipase (pRML) in Pichia pastoris X-33 via propeptide mutation and high-copy strain screening. The mutated enzyme was then used to simultaneously catalyze the production of both biodiesel and EPA-EE. The P46N mutation in the propeptide (P46N-pRML) significantly boosted its production, with the four-copy strain increasing enzyme yield by 3.7-fold, reaching 3425 U/mL. Meanwhile, its optimal temperature increased to 45-50 degreesC, pH expanded to 7.0-8.0, specific activity doubled, K(m) reduced to one-third, and k(cat)/K(m) increased 7-fold. Notably, P46N-pRML efficiently converts Nannochloropsis gaditana oil's eicosapentaenoic acid (EPA). Under optimal conditions, it achieves up to 93% biodiesel and 92% EPA-EE yields in 9 h. Our study introduces a novel, efficient one-step green method to produce both biodiesel and EPA-EE using this advanced enzyme.

PubMedSearch : Lu_2024_J.Agric.Food.Chem__
PubMedID: 39028993

Related information

Citations formats

Lu T, Qian Y, Zhu Y, Ju X, Dai W, Xu Q, Yang Q, Li S, Yuan B, Huang J (2024)
Efficient Expression and Application of a Modified Rhizomucor miehei Lipase for Simultaneous Production of Biodiesel and Eicosapentaenoic Acid Ethyl Ester from Nannochloropsis Oil
Journal of Agricultural and Food Chemistry :

Lu T, Qian Y, Zhu Y, Ju X, Dai W, Xu Q, Yang Q, Li S, Yuan B, Huang J (2024)
Journal of Agricultural and Food Chemistry :