Ludwig_2003_Biochem.Biophys.Res.Commun_304_73

Reference

Title : The 3D structure of rat DPPIV\/CD26 as obtained by cryo-TEM and single particle analysis - Ludwig_2003_Biochem.Biophys.Res.Commun_304_73
Author(s) : Ludwig K , Yan S , Fan H , Reutter W , Bottcher C
Ref : Biochemical & Biophysical Research Communications , 304 :73 , 2003
Abstract :

We present the three-dimensional structure of rat DPPIV/CD26, as determined by cryo-TEM and single particle analysis at a resolution of approximately 14A. The reconstruction confirms that the protein exists as a dimer, as predicted earlier. Since there are structural analogies to the serine peptidase POP, docking calculations of the two structures were performed. Although the docking showed a similar spatial organization (catalytic domain, beta-propeller, distal opening, central cavity), the detailed comparison revealed clear discrepancies. The most marked difference is a second (lateral) opening in DPPIV/CD26, which would enable direct access to the catalytic site. We therefore assume that substrate selectivity and binding rate are most probably driven by different mechanisms in DPPIV/CD26 and POP.

PubMedSearch : Ludwig_2003_Biochem.Biophys.Res.Commun_304_73
PubMedID: 12705886
Gene_locus related to this paper: ratno-dpp4

Related information

Gene_locus ratno-dpp4

Citations formats

Ludwig K, Yan S, Fan H, Reutter W, Bottcher C (2003)
The 3D structure of rat DPPIV\/CD26 as obtained by cryo-TEM and single particle analysis
Biochemical & Biophysical Research Communications 304 :73

Ludwig K, Yan S, Fan H, Reutter W, Bottcher C (2003)
Biochemical & Biophysical Research Communications 304 :73