| Title : Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors - Lyukmanova_2016_Toxicon_119_274 |
| Author(s) : Lyukmanova EN , Shulepko MA , Shenkarev ZO , Kasheverov IE , Chugunov AO , Kulbatskii DS , Myshkin MY , Utkin YN , Efremov RG , Tsetlin VI , Arseniev AS , Kirpichnikov MP , Dolgikh DA |
| Ref : Toxicon , 119 :274 , 2016 |
|
Abstract :
'Three-finger' toxin WTX from Naja kaouthia interacts with nicotinic and muscarinic acetylcholine receptors (nAChRs and mAChRs). Mutagenesis and competition experiments with (125)I-alpha-bungarotoxin revealed that Arg31 and Arg32 residues from the WTX loop II are important for binding to Torpedo californica and human alpha7 nAChRs. Computer modeling suggested that loop II occupies the orthosteric binding site at alpha7 nAChR. The similar toxin interface was previously described as a major determinant of allosteric interactions with mAChRs. |
| PubMedSearch : Lyukmanova_2016_Toxicon_119_274 |
| PubMedID: 27343701 |
Lyukmanova EN, Shulepko MA, Shenkarev ZO, Kasheverov IE, Chugunov AO, Kulbatskii DS, Myshkin MY, Utkin YN, Efremov RG, Tsetlin VI, Arseniev AS, Kirpichnikov MP, Dolgikh DA (2016)
Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors
Toxicon
119 :274
Lyukmanova EN, Shulepko MA, Shenkarev ZO, Kasheverov IE, Chugunov AO, Kulbatskii DS, Myshkin MY, Utkin YN, Efremov RG, Tsetlin VI, Arseniev AS, Kirpichnikov MP, Dolgikh DA (2016)
Toxicon
119 :274