| Title : Deletion of bem46 retards spore germination and may be related to the polar growth of Aspergillus fumigatus - Ma_2020_Med.Mycol_58_690 |
| Author(s) : Ma Y , Ji Y , Yang J , Li W , Li J , Cen W , Wang Y , Feng W |
| Ref : Med Mycol , 58 :690 , 2020 |
| Abstract : Bud emergence 46 (BEM46), a member of the alpha/beta hydrolase superfamily, has been reported to be essential for polarized growth in Neurospora crassa. However, the role of BEM46 in aspergillus fumigatus (A. fumigatus) remains unclear. In this study, we constructed an A. fumigatus strain expressing BEM46 fused with enhanced green fluorescent protein, and a Deltabem46 mutant, to explore the localization and the role of growth of BEM46 in A. fumigatus, respectively. Confocal laser scanning microscopy revealed that BEM46 was dominantly expressed in the sites where hyphae germinated from conidia in A. fumigatus. When compared with the control strain, the Deltabem46 mutant exhibited insignificant morphological changes but delayed germination. No significant changes were found regarding the radial growth of both strains in response to various antifungal agents. These results suggest that BEM46 plays an essential role in timely germination in A. fumigatus. From the observation of fluorescence localization, we infer that that BEM46 might be involved in polarized growth in A. fumigatus. |
| ESTHER : Ma_2020_Med.Mycol_58_690 |
| PubMedSearch : Ma_2020_Med.Mycol_58_690 |
| PubMedID: 31711175 |
| Gene_locus related to this paper: aspfu-q4wgd5 |
| Gene_locus related to this paper: aspfu-q4wgd5 |
Ma Y, Ji Y, Yang J, Li W, Li J, Cen W, Wang Y, Feng W (2020)
Deletion of bem46 retards spore germination and may be related to the polar growth of Aspergillus fumigatus
Med Mycol
58 :690
Ma Y, Ji Y, Yang J, Li W, Li J, Cen W, Wang Y, Feng W (2020)
Med Mycol
58 :690