| Title : PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses Paracoccus denitrificans phaP expression in vitro - Maehara_2001_FEMS.Microbiol.Lett_200_9 |
| Author(s) : Maehara A , Doi Y , Nishiyama T , Takagi Y , Ueda S , Nakano H , Yamane T |
| Ref : FEMS Microbiology Letters , 200 :9 , 2001 |
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Abstract :
A putative regulatory protein, PhaR, which was identified in the polyhydroxyalkanoic acid synthetic locus (phaZCPR) in Paracoccus denitrificans, was investigated. The PhaR protein purified from a recombinant Escherichia coli was estimated to be 22 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, being consistent with the mass calculated from the nucleotide sequence. The molecular mass was determined to be 93 kDa by size-exclusion chromatography, suggesting that the protein formed a tetramer. A gel mobility shift assay showed that PhaR specifically bound to the intergenic region of phaC--phaP. In a cell-free protein synthesis system using E. coli S30 extract, the expression of the phaP gene was repressed by the addition of purified PhaR. These results suggest that PhaR is a DNA-binding protein and may play a role in the regulation of phaP gene expression. |
| PubMedSearch : Maehara_2001_FEMS.Microbiol.Lett_200_9 |
| PubMedID: 11410342 |
| Gene_locus related to this paper: parde-PHAC , parde-Q9WX79 |
| Gene_locus | parde-PHAC parde-Q9WX79 |
Maehara A, Doi Y, Nishiyama T, Takagi Y, Ueda S, Nakano H, Yamane T (2001)
PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses Paracoccus denitrificans phaP expression in vitro
FEMS Microbiology Letters
200 :9
Maehara A, Doi Y, Nishiyama T, Takagi Y, Ueda S, Nakano H, Yamane T (2001)
FEMS Microbiology Letters
200 :9