Major_2020_Evol.Appl_13_620

Reference

Title : The G119S ace-1 mutation confers adaptive organophosphate resistance in a nontarget amphipod - Major_2020_Evol.Appl_13_620
Author(s) : Major KM , Weston DP , Lydy MJ , Huff Hartz KE , Wellborn GA , Manny AR , Poynton HC
Ref : Evol Appl , 13 :620 , 2020
Abstract :

Organophosphate (OP) and carbamate (CM) insecticides are widely used in the United States and share the same mode of toxic action. Both classes are frequently documented in aquatic ecosystems, sometimes at levels that exceed aquatic life benchmarks. We previously identified a population of the nontarget amphipod, Hyalella azteca, thriving in an agricultural creek with high sediment levels of the OP chlorpyrifos, suggesting the population may have acquired genetic resistance to the pesticide. In the present study, we surveyed 17 populations of H. azteca in California to screen for phenotypic resistance to chlorpyrifos as well as genetic signatures of resistance in the acetylcholinesterase (ace-1) gene. We found no phenotypic chlorpyrifos resistance in populations from areas with little or no pesticide use. However, there was ~3- to 1,000-fold resistance in H. azteca populations from agricultural and/or urban areas, with resistance levels in agriculture being far higher than urban areas due to greater ongoing use of OP and CM pesticides. In every case of resistance in H. azteca, we identified a glycine-to-serine amino acid substitution (G119S) that has been shown to confer OP and CM resistance in mosquitoes and has been associated with resistance in other insects. We found that the G119S mutation was always present in a heterozygous state. Further, we provide tentative evidence of an ace-1 gene duplication in H. azteca that may play a role in chlorpyrifos resistance in some populations. The detection of a genetically based, adaptive OP and CM resistance in some of the same populations of H. azteca previously shown to harbor a genetically based adaptive pyrethroid resistance indicates that these nontarget amphipod populations have become resistant to many of the insecticides now in common use. The terrestrial application of pesticides has provided strong selective pressures to drive evolution in a nontarget, aquatic species.

PubMedSearch : Major_2020_Evol.Appl_13_620
PubMedID: 32211056
Gene_locus related to this paper: hyaaz-ACHE1

Related information

Mutation G119S_hyaaz-ACHE1
Gene_locus G119S_hyaaz-ACHE1    hyaaz-ACHE1

Citations formats

Major KM, Weston DP, Lydy MJ, Huff Hartz KE, Wellborn GA, Manny AR, Poynton HC (2020)
The G119S ace-1 mutation confers adaptive organophosphate resistance in a nontarget amphipod
Evol Appl 13 :620

Major KM, Weston DP, Lydy MJ, Huff Hartz KE, Wellborn GA, Manny AR, Poynton HC (2020)
Evol Appl 13 :620