Makela_2018_Microb.Biotechnol_11_869

Reference

Title : Characterization of a feruloyl esterase from Aspergillus terreus facilitates the division of fungal enzymes from Carbohydrate Esterase family 1 of the carbohydrate-active enzymes (CAZy) database - Makela_2018_Microb.Biotechnol_11_869
Author(s) : Makela MR , Dilokpimol A , Koskela SM , Kuuskeri J , de Vries RP , Hilden K
Ref : Microb Biotechnol , 11 :869 , 2018
Abstract :

Feruloyl esterases (FAEs) are accessory enzymes for plant biomass degradation, which catalyse hydrolysis of carboxylic ester linkages between hydroxycinnamic acids and plant cell-wall carbohydrates. They are a diverse group of enzymes evolved from, e.g. acetyl xylan esterases (AXEs), lipases and tannases, thus complicating their classification and prediction of function by sequence similarity. Recently, an increasing number of fungal FAEs have been biochemically characterized, owing to their potential in various biotechnological applications and multitude of candidate FAEs in fungal genomes. However, only part of the fungal FAEs are included in Carbohydrate Esterase family 1 (CE1) of the carbohydrate-active enzymes (CAZy) database. In this work, we performed a phylogenetic analysis that divided the fungal members of CE1 into five subfamilies of which three contained characterized enzymes with conserved activities. Conservation within one of the subfamilies was confirmed by characterization of an additional CE1 enzyme from Aspergillus terreus. Recombinant A. terreus FaeD (AtFaeD) showed broad specificity towards synthetic methyl and ethyl esters, and released ferulic acid from plant biomass substrates, demonstrating its true FAE activity and interesting features as potential biocatalyst. The subfamily division of the fungal CE1 members enables more efficient selection of candidate enzymes for biotechnological processes.

PubMedSearch : Makela_2018_Microb.Biotechnol_11_869
PubMedID: 29697197
Gene_locus related to this paper: asptn-q0ci40

Citations formats

Makela MR, Dilokpimol A, Koskela SM, Kuuskeri J, de Vries RP, Hilden K (2018)
Characterization of a feruloyl esterase from Aspergillus terreus facilitates the division of fungal enzymes from Carbohydrate Esterase family 1 of the carbohydrate-active enzymes (CAZy) database
Microb Biotechnol 11 :869

Makela MR, Dilokpimol A, Koskela SM, Kuuskeri J, de Vries RP, Hilden K (2018)
Microb Biotechnol 11 :869