| Title : Separation and purification of bioactive botrallin and TMC-264 by a combination of HSCCC and semi-preparative HPLC from endophytic fungus Hyalodendriella sp. Ponipodef12 - Mao_2014_World.J.Microbiol.Biotechnol_30_2533 |
| Author(s) : Mao Z , Luo R , Luo H , Tian J , Liu H , Yue Y , Wang M , Peng Y , Zhou L |
| Ref : World J Microbiol Biotechnol , 30 :2533 , 2014 |
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Abstract :
Two dibenzo-alpha-pyrones, botrallin (1) and TMC-264 (2) were preparatively separated from crude ethyl acetate extract of the endophytic fungus Hyalodendriella sp. Ponipodef12, which was isolated from the hybrid 'Neva' of Populus deltoides Marsh x P. nigra L. using a combination of high-speed counter-current chromatography (HSCCC) and semi-preparative HPLC. Botrallin (1) with 74.73 % of purity and TMC-264 (2) with 82.29 % of purity were obtained through HSCCC by employing a solvent system containing n-hexane-ethyl acetate-methanol-water at a volume ratio of 1.2:1.0:0.9:1.0. It was the first time for TMC-264 (2) to be isolated from this fungus. TMC-264 (2) showed strong antimicrobial and antinematodal activity, and botrallin (1) exhibited moderate inhibitory activity on acetylcholinesterase. |
| PubMedSearch : Mao_2014_World.J.Microbiol.Biotechnol_30_2533 |
| PubMedID: 24898177 |
Mao Z, Luo R, Luo H, Tian J, Liu H, Yue Y, Wang M, Peng Y, Zhou L (2014)
Separation and purification of bioactive botrallin and TMC-264 by a combination of HSCCC and semi-preparative HPLC from endophytic fungus Hyalodendriella sp. Ponipodef12
World J Microbiol Biotechnol
30 :2533
Mao Z, Luo R, Luo H, Tian J, Liu H, Yue Y, Wang M, Peng Y, Zhou L (2014)
World J Microbiol Biotechnol
30 :2533