| Title : The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene - Marcusson_1994_Cell_77_579 |
| Author(s) : Marcusson EG , Horazdovsky BF , Cereghino JL , Gharakhanian E , Emr SD |
| Ref : Cell , 77 :579 , 1994 |
| Abstract : The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY). Mutations in VPS10 result in the selective missorting and secretion of CPY; all other vacuolar proteins tested are delivered to the vacuole in vps10 mutants. Chemical cross-linking studies demonstrate that Vps10p and the Golgi-modified precursor form of CPY directly interact. A single amino acid change in the CPY vacuolar sorting signal prevents this interaction. Vps10p also interacts with a hybrid protein containing the CPY sorting signal fused to the normally secreted enzyme invertase. Subcellular fractionation indicates that the majority of Vps10p is localized to a late Golgi compartment where vacuolar proteins are sorted. We propose that VPS10 encodes a CPY sorting receptor that executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. |
| ESTHER : Marcusson_1994_Cell_77_579 |
| PubMedSearch : Marcusson_1994_Cell_77_579 |
| PubMedID: 8187177 |
| Gene_locus related to this paper: yeast-cbpy1 |
| Gene_locus related to this paper: yeast-cbpy1 |
Marcusson EG, Horazdovsky BF, Cereghino JL, Gharakhanian E, Emr SD (1994)
The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene
Cell
77 :579
Marcusson EG, Horazdovsky BF, Cereghino JL, Gharakhanian E, Emr SD (1994)
Cell
77 :579