yeast-cbpy1

Saccharomyces cerevisiae Carboxypeptidase Y, vacuolar PRC1 gene encoding preproprotein carboxypeptidase Y (CPY)

Comment

Other strains: Saccharomyces cerevisiae (AWRI1631\; Lalvin EC1118 \/ Prise de mousse\; Lalvin QA23\; RM11-1a\; VIN 13\; YJM789\; Zymaflore VL3\; JAY291\; Kyokai no. 7 \/ NBRC 10155\; VIN7) (Baker's yeast)

Relationship

Family : Carboxypeptidase_S10

Block : X

Position in NCBI Life Tree : Saccharomyces cerevisiae

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Opisthokonta N E > Fungi N E > Dikarya N E > Ascomycota N E > saccharomyceta N E > Saccharomycotina N E > Saccharomycetes N E > Saccharomycetales N E > Saccharomycetaceae N E > Saccharomyces N E > Saccharomyces cerevisiae N E

Molecular evidence

No mutation

No kinetic

No disease

No substrate

No inhibitor

Sequence

Peptide

MKAFTSLLCG LGLSTTLAKA ISLQRPLGLD KDVLLQAAEK FGLDLDLDHL LKELDSNVLD AWAQIEHLYP NQVMSLETST KPKFPEAIKT KKDWDFVVKN DAIENYQLRV NKIKDPKILG IDPNVTQYTG YLDVEDEDKH FFFWTFESRN DPAKDPVILW LNGGPGCSSL TGLFFELGPS SIGPDLKPIG NPYSWNSNAT VIFLDQPVNV GFSYSGSSGV SNTVAAGKDV YNFLELFFDQ FPEYVNKGQD FHIAGESYAG HYIPVFASEI LSHKDRNFNL TSVLIGNGLT DPLTQYNYYE PMACGEGGEP SVLPSEECSA MEDSLERCLG LIESCYDSQS VWSCVPATIY CNNAQLAPYQ RTGRNVYDIR KDCEGGNLCY PTLQDIDDYL NQDYVKEAVG AEVDHYESCN FDINRNFLFA GDWMKPYHTA VTDLLNQDLP ILVYAGDKDF ICNWLGNKAW TDVLPWKYDE EFASQKVRNW TASITDEVAG EVKSYKHFTY LRVFNGGHMV PFDVPENALS MVNEWIHGGF SL

References (42)

Title : Carboxypeptidase Y activity and maintenance is modulated by a large helical structure - Makino_2019_FEBS.Open.Bio_9_1337
Author(s) : Makino M , Sahara T , Morita N , Ueno H
Ref : FEBS Open Bio , 9 :1337 , 2019
PubMedID: 31173671
Gene_locus related to this paper: yeast-cbpy1

Title : Eukaryote-to-eukaryote gene transfer events revealed by the genome sequence of the wine yeast Saccharomyces cerevisiae EC1118 - Novo_2009_Proc.Natl.Acad.Sci.U.S.A_106_16333
Author(s) : Novo M , Bigey F , Beyne E , Galeote V , Gavory F , Mallet S , Cambon B , Legras JL , Wincker P , Casaregola S , Dequin S
Ref : Proc Natl Acad Sci U S A , 106 :16333 , 2009
PubMedID: 19805302
Gene_locus related to this paper: yeast-AIM2 , yeast-BST1 , yeast-cbpy1 , yeast-cld1 , yeast-dap1 , yeast-dap2 , yeast-dlhh , yeast-ECM18 , yeast-FSH1 , yeast-FSH3 , yeast-ict1 , yeast-kex01 , yeast-MCFS1 , yeast-MCFS2 , yeast-met2 , yeast-mgll , yeast-pdat , yeast-ppme1 , yeast-ROG1 , yeast-SAY1 , yeast-tgl1 , yeast-tgl2 , yeast-yby9 , yeast-YDL109C , yeast-YDR428C , yeast-YDR444W , yeast-yg19 , yeast-yj77 , yeast-yjg8 , yeast-YLL012W , yeast-YLR020C , yeast-YLR118c , yeast-ym60 , yeast-ynl5 , yeast-yo059 , yeast-YPR147C , yeast-hda1

Title : Structure of the carboxypeptidase Y inhibitor IC in complex with the cognate proteinase reveals a novel mode of the proteinase-protein inhibitor interaction - Mima_2005_J.Mol.Biol_346_1323
Author(s) : Mima J , Hayashida M , Fujii T , Narita Y , Hayashi R , Ueda M , Hata Y
Ref : Journal of Molecular Biology , 346 :1323 , 2005
PubMedID: 15713484
Gene_locus related to this paper: yeast-cbpy1

Title : Crystallization and preliminary X-ray analysis of carboxypeptidase Y inhibitor IC complexed with the cognate proteinase - Mima_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_1622
Author(s) : Mima J , Hayashida M , Fujii T , Hata Y , Hayashi R , Ueda M
Ref : Acta Crystallographica D Biol Crystallogr , 60 :1622 , 2004
PubMedID: 15333936
Gene_locus related to this paper: yeast-cbpy1

Title : The multiple site binding of carboxypeptidase Y inhibitor (IC) to the cognate proteinase. Implications for the biological roles of the phosphatidylethanolamine-binding protein - Mima_2003_J.Biol.Chem_278_29792
Author(s) : Mima J , Narita Y , Chiba H , Hayashi R
Ref : Journal of Biological Chemistry , 278 :29792 , 2003
PubMedID: 12791700
Gene_locus related to this paper: yeast-cbpy1

Title : Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae - Mima_2002_J.Biochem_132_967
Author(s) : Mima J , Suzuki H , Takahashi M , Hayashi R
Ref : J Biochem , 132 :967 , 2002
PubMedID: 12473200
Gene_locus related to this paper: yeast-cbpy1

Title : N-terminal acetyl group is essential for the inhibitory function of carboxypeptidase Y inhibitor (I(C)) - Mima_2002_FEBS.Lett_532_207
Author(s) : Mima J , Kondo T , Hayashi R
Ref : FEBS Letters , 532 :207 , 2002
PubMedID: 12459491
Gene_locus related to this paper: yeast-cbpy1

Title : Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper - Maki_2002_Biosci.Biotechnol.Biochem_66_1393
Author(s) : Maki T , Kozawa H , Mima J , Ueno H , Hayashi R
Ref : Biosci Biotechnol Biochem , 66 :1393 , 2002
PubMedID: 12162566
Gene_locus related to this paper: yeast-cbpy1

Title : Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y - Mima_2002_Eur.J.Biochem_269_3220
Author(s) : Mima J , Jung G , Onizuka T , Ueno H , Hayashi R
Ref : European Journal of Biochemistry , 269 :3220 , 2002
PubMedID: 12084062
Gene_locus related to this paper: yeast-cbpy1

Title : Refolding and purification of yeast carboxypeptidase Y expressed as inclusion bodies in Escherichia coli - Hahm_2001_Protein.Expr.Purif_22_101
Author(s) : Hahm MS , Chung BH
Ref : Protein Expr Purif , 22 :101 , 2001
PubMedID: 11388806
Gene_locus related to this paper: yeast-cbpy1

Title : Carboxypeptidase Y: structural basis for protein sorting and catalytic triad - Jung_1999_J.Biochem_126_1
Author(s) : Jung G , Ueno H , Hayashi R
Ref : J Biochem , 126 :1 , 1999
PubMedID: 10393313
Gene_locus related to this paper: yeast-cbpy1

Title : A high-affinity inhibitor of yeast carboxypeptidase Y is encoded by TFS1 and shows homology to a family of lipid binding proteins - Bruun_1998_Biochemistry_37_3351
Author(s) : Bruun AW , Svendsen I , Sorensen SO , Kielland-Brandt MC , Winther JR
Ref : Biochemistry , 37 :3351 , 1998
PubMedID: 9521655
Gene_locus related to this paper: yeast-cbpy1

Title : Reversed-flow affinity elution applied to the purification of carboxypeptidase Y - Mortensen_1998_Anal.Biochem_258_236
Author(s) : Mortensen UH , Stennicke HR , Breddam K
Ref : Analytical Biochemistry , 258 :236 , 1998
PubMedID: 9570835
Gene_locus related to this paper: yeast-cbpy1

Title : The specificity of carboxypeptidase Y may be altered by changing the hydrophobicity of the S'1 binding pocket - Sorensen_1997_Protein.Sci_6_2227
Author(s) : Sorensen SB , Breddam K
Ref : Protein Science , 6 :2227 , 1997
PubMedID: 9336845
Gene_locus related to this paper: yeast-cbpy1

Title : Substrates with charged P1 residues are efficiently hydrolyzed by serine carboxypeptidases when S3-P1 interactions are facilitated - Olesen_1997_Biochemistry_36_12235
Author(s) : Olesen K , Breddam K
Ref : Biochemistry , 36 :12235 , 1997
PubMedID: 9315861
Gene_locus related to this paper: yeast-cbpy1

Title : Exchange of regions of the carboxypeptidase Y propeptide. Sequence specificity and function in folding in vivo - Ramos_1996_Eur.J.Biochem_242_29
Author(s) : Ramos C , Winther JR
Ref : European Journal of Biochemistry , 242 :29 , 1996
PubMedID: 8954149
Gene_locus related to this paper: yeast-cbpy1

Title : Site-Directed Mutagenesis on (Serine) Carboxypeptidase Y from Yeast. The Significance of Thr60 and Met398 in Hydrolysis and Aminolysis Reactions - Sorensen_1995_J.Am.Chem.Soc_117_5944
Author(s) : Sorensen SB , Raaschou-Nielsen M , Mortensen UH , Remington SJ , Breddam K
Ref : Journal of the American Chemical Society , 117 :5944 , 1995
PubMedID:
Gene_locus related to this paper: yeast-cbpy1

Title : Identification of the catalytic histidine residue participating in the charge-relay system of carboxypeptidase Y - Jung_1995_Protein.Sci_4_2433
Author(s) : Jung G , Ueno H , Hayashi R , Liao TH
Ref : Protein Science , 4 :2433 , 1995
PubMedID: 8563642
Gene_locus related to this paper: yeast-cbpy1

Title : 2.8-A structure of yeast serine carboxypeptidase - Endrizzi_1994_Biochemistry_33_11106
Author(s) : Endrizzi JA , Breddam K , Remington SJ
Ref : Biochemistry , 33 :11106 , 1994
PubMedID: 7727362
Gene_locus related to this paper: yeast-cbpy1

Title : The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene - Marcusson_1994_Cell_77_579
Author(s) : Marcusson EG , Horazdovsky BF , Cereghino JL , Gharakhanian E , Emr SD
Ref : Cell , 77 :579 , 1994
PubMedID: 8187177
Gene_locus related to this paper: yeast-cbpy1

Title : A conserved glutamic acid bridge in serine carboxypeptidases, belonging to the alpha\/beta hydrolase fold, acts as a pH-dependent protein-stabilizing element - Mortensen_1994_Protein.Sci_3_838
Author(s) : Mortensen UH , Breddam K
Ref : Protein Science , 3 :838 , 1994
PubMedID: 7914789
Gene_locus related to this paper: yeast-cbpy1

Title : Refolding of a carboxypeptidase Y folding intermediate in vitro by low-affinity binding of the proregion - Winther_1994_J.Biol.Chem_269_22007
Author(s) : Winther JR , Sorensen P , Kielland-Brandt MC
Ref : Journal of Biological Chemistry , 269 :22007 , 1994
PubMedID: 8071321
Gene_locus related to this paper: yeast-cbpy1

Title : Active-site residues of procarboxypeptidase Y are accessible to chemical modification - Sorensen_1994_Biochim.Biophys.Acta_1205_289
Author(s) : Sorensen SO , Winther JR
Ref : Biochimica & Biophysica Acta , 1205 :289 , 1994
PubMedID: 8155711
Gene_locus related to this paper: yeast-cbpy1

Title : pH-dependent processing of yeast procarboxypeptidase Y by proteinase A in vivo and in vitro - Sorensen_1994_Eur.J.Biochem_220_19
Author(s) : Sorensen SO , van den Hazel HB , Kielland-Brandt MC , Winther JR
Ref : European Journal of Biochemistry , 220 :19 , 1994
PubMedID: 8119286
Gene_locus related to this paper: yeast-cbpy1

Title : Effects of introduced aspartic and glutamic acid residues on the P'1 substrate specificity, pH dependence and stability of carboxypeptidase Y - Stennicke_1994_Protein.Eng_7_911
Author(s) : Stennicke HR , Mortensen UH , Christensen U , Remington SJ , Breddam K
Ref : Protein Engineering , 7 :911 , 1994
PubMedID: 7971953
Gene_locus related to this paper: yeast-cbpy1

Title : Recognition of C-terminal amide groups by (serine) carboxypeptidase Y investigated by site-directed mutagenesis - Mortensen_1994_J.Biol.Chem_269_15528
Author(s) : Mortensen UH , Raaschou-Nielsen M , Breddam K
Ref : Journal of Biological Chemistry , 269 :15528 , 1994
PubMedID: 8195197
Gene_locus related to this paper: yeast-cbpy1

Title : Improvement of the applicability of carboxypeptidase Y in peptide synthesis by protein engineering - Raaschou-Nielsen_1994_Pept.Res_7_132
Author(s) : Raaschou-Nielsen M , Mortensen UH , Olesen K , Breddam K
Ref : Pept Res , 7 :132 , 1994
PubMedID: 8081068
Gene_locus related to this paper: yeast-cbpy1

Title : Site-directed mutagenesis on (serine) carboxypeptidase Y. A hydrogen bond network stabilizes the transition state by interaction with the C-terminal carboxylate group of the substrate - Mortensen_1994_Biochemistry_33_508
Author(s) : Mortensen UH , Remington SJ , Breddam K
Ref : Biochemistry , 33 :508 , 1994
PubMedID: 7904479
Gene_locus related to this paper: yeast-cbpy1

Title : The pro region required for folding of carboxypeptidase Y is a partially folded domain with little regular structural core - Sorenson_1993_Biochemistry_32_12160
Author(s) : Sorenson P , Winther JR , Kaarsholm NC , Poulsen FM
Ref : Biochemistry , 32 :12160 , 1993
PubMedID: 8218293
Gene_locus related to this paper: yeast-cbpy1

Title : Application of quantitative structure-activity relationship modeling to the evaluation of the changes in enzymatic activity of carboxypeptidase Y upon chemical modifications - Kanstrup_1993_Arch.Biochem.Biophys_304_332
Author(s) : Kanstrup A , Breddam K , Buchardt O
Ref : Archives of Biochemistry & Biophysics , 304 :332 , 1993
PubMedID: 8346910
Gene_locus related to this paper: yeast-cbpy1

Title : C-terminal amidation of calcitonin by carboxypeptidase Y catalyzed transpeptidation with a photocleavable nucleophile - Henriksen_1992_Pept.Res_5_321
Author(s) : Henriksen DB , Rolland M , Jakobsen MH , Buchardt O , Breddam K
Ref : Pept Res , 5 :321 , 1992
PubMedID: 1493358
Gene_locus related to this paper: yeast-cbpy1

Title : Propeptide of carboxypeptidase Y provides a chaperone-like function as well as inhibition of the enzymatic activity - Winther_1991_Proc.Natl.Acad.Sci.U.S.A_88_9330
Author(s) : Winther JR , Sorensen P
Ref : Proc Natl Acad Sci U S A , 88 :9330 , 1991
PubMedID: 1924396
Gene_locus related to this paper: yeast-cbpy1

Title : Regulated overproduction and secretion of yeast carboxypeptidase Y - Nielsen_1990_Appl.Microbiol.Biotechnol_33_307
Author(s) : Nielsen TL , Holmberg S , Petersen JG
Ref : Applied Microbiology & Biotechnology , 33 :307 , 1990
PubMedID: 1366639
Gene_locus related to this paper: yeast-cbpy1

Title : Inactivation of carboxypeptidase Y by mutational removal of the putative essential histidyl residue - Bech_1989_Carlsberg.Res.Commun_54_165
Author(s) : Bech LM , Breddam K
Ref : Carlsberg Res Commun , 54 :165 , 1989
PubMedID: 2639680
Gene_locus related to this paper: yeast-cbpy1

Title : Chemical modifications of a cysteinyl residue introduced in the binding site of carboxypeptidase Y by site-directed mutagenesis - Bech_1988_Carlsberg.Res.Commun_53_381
Author(s) : Bech LM , Breddam K
Ref : Carlsberg Res Commun , 53 :381 , 1988
PubMedID: 3255314
Gene_locus related to this paper: yeast-cbpy1

Title : Protein sorting in yeast: the localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide - Valls_1987_Cell_48_887
Author(s) : Valls LA , Hunter CP , Rothman JH , Stevens TH
Ref : Cell , 48 :887 , 1987
PubMedID: 3028649
Gene_locus related to this paper: yeast-cbpy1

Title : Studies on the carboxypeptidase Y-inhibitor complex of yeast - Barth_1978_Biochim.Biophys.Acta_527_63
Author(s) : Barth R , Wolf DH , Holzer H
Ref : Biochimica & Biophysica Acta , 527 :63 , 1978
PubMedID: 363166
Gene_locus related to this paper: yeast-cbpy1

Title : Further confirmation of carboxypeptidase Y as a metal-free enzyme having a reactive serine residue - Hayashi_1975_J.Biochem_77_1313
Author(s) : Hayashi R , Bai Y , Hata T
Ref : J Biochem , 77 :1313 , 1975
PubMedID: 5404
Gene_locus related to this paper: yeast-cbpy1

Title : Serine at the active center of yeast carboxypeptidase -
Author(s) : Hayashi R , Moore S , Stein WH
Ref : Journal of Biological Chemistry , 248 :8366 , 1973
PubMedID: 4587122
Gene_locus related to this paper: yeast-cbpy1