| Title : Cloning and molecular characterization of the citrate utilization citMCDEFGRP cluster of Leuconostoc paramesenteroides - Martin_1999_FEMS.Microbiol.Lett_174_231 |
| Author(s) : Martin M , Corrales MA , de Mendoza D , Lopez P , Magni C |
| Ref : FEMS Microbiology Letters , 174 :231 , 1999 |
|
Abstract :
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF the subunits of the citrate lyase of Leuconostoc. Moreover, it suggests that citM encodes a Leuconostoc malic enzyme. Analysis of citrate consumption by and citrate lyase activity of Lc. paramesenteroides J1[pCITJ1] showed that its citrate permease and its citrate lyase are induced by the presence of citrate in the growth medium. Southern blot analysis demonstrated that the citMCDEFGRP cluster is located in a plasmid. |
| PubMedSearch : Martin_1999_FEMS.Microbiol.Lett_174_231 |
| PubMedID: 10339813 |
| Gene_locus related to this paper: weipa-citR |
| Gene_locus | weipa-citR |
Martin M, Corrales MA, de Mendoza D, Lopez P, Magni C (1999)
Cloning and molecular characterization of the citrate utilization citMCDEFGRP cluster of Leuconostoc paramesenteroides
FEMS Microbiology Letters
174 :231
Martin M, Corrales MA, de Mendoza D, Lopez P, Magni C (1999)
FEMS Microbiology Letters
174 :231