| Title : Expression of recombinant human acetylcholinesterase in transgenic tomato plants - Mor_2001_Biotechnol.Bioeng_75_259 |
| Author(s) : Mor TS , Sternfeld M , Soreq H , Arntzen CJ , Mason HS |
| Ref : Biotechnol Bioeng , 75 :259 , 2001 |
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Abstract :
Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min(-1) mg protein(-1)) and fruits (up to 250 nmol min(-1) mg protein(-1)). |
| PubMedSearch : Mor_2001_Biotechnol.Bioeng_75_259 |
| PubMedID: 11590598 |
Mor TS, Sternfeld M, Soreq H, Arntzen CJ, Mason HS (2001)
Expression of recombinant human acetylcholinesterase in transgenic tomato plants
Biotechnol Bioeng
75 :259
Mor TS, Sternfeld M, Soreq H, Arntzen CJ, Mason HS (2001)
Biotechnol Bioeng
75 :259